LOCUS       sequence-248373-        3509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part O- ORF; araC; transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-araC_O
FEATURES             Location/Qualifiers
     source          1..3509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             83..961
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="AEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGYI
                     LNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYWH
                     EWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRRM
                     EAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGISV
                     LSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCEE
                     KVNDVAVKLSS"
     misc_feature    979..999
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1033..1127
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1200..1291
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1292..1360,1361..2152)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2156..2195
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2342..2450
                     /note="incP origin of transfer"
     rep_origin      2486..3074
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2975..2994
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3228..3245
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3362..3383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3417..3503)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tagccgaagc gcaaaatgat cccctgctgc cgggatactc
      121 gtttaatgcc catctggtgg cgggtttaac gccgattgag gccaacggtt atctcgattt
      181 ttttatcgac cgaccgctgg gaatgaaagg ttatattctc aatctcacca ttcgcggtca
      241 gggggtggtg aaaaatcagg gacgagaatt tgtctgccga ccgggtgata ttttgctgtt
      301 cccgccagga gagattcatc actacggtcg tcatccggag gctcgcgaat ggtatcacca
      361 gtgggtttac tttcgtccgc gcgcctactg gcatgaatgg cttaactggc cgtcaatatt
      421 tgccaatacg ggtttctttc gcccggatga agcgcaccag ccgcatttca gcgacctgtt
      481 tgggcaaatc attaacgccg ggcaagggga agggcgctat tcggagctgc tggcgataaa
      541 tctgcttgag caattgttac tgcggcgcat ggaagcgatt aacgagtcgc tccatccacc
      601 gatggataat cgggtacgcg aggcttgtca gtacatcagc gatcacctgg cagacagcaa
      661 ttttgatatc gccagcgtcg cacagcatgt ttgcttgtcg ccgtcgcgtc tgtcacatct
      721 tttccgccag cagttaggga ttagcgtctt aagctggcgc gaggaccaac gtatcagcca
      781 ggcgaagctg cttttgagca ctacccggat gcctatcgcc accgtcggtc gcaatgttgg
      841 ttttgacgat caactctatt tctcgcgggt atttaaaaaa tgcaccgggg ccagcccgag
      901 cgagttccgt gccggttgtg aagaaaaagt gaatgatgta gccgtcaagt tatcttcgtg
      961 agacctttca tcgcgaccta ctagtagcgg ccgctgcagg gagttgtctt cgaagacttc
     1021 gctctagtct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
     1081 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca ggggtcccca
     1141 ataattacga tttaaattag tagcccgcct aatgagcggg ctttttttta attcccctat
     1201 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1261 aatgcttcaa taatattgaa aaaggaagag tatgagcatt cagcattttc gtgtggcgct
     1321 gattccgttt tttgcggcgt tttgcctgcc ggtgtttgcg catccggaaa ccctggtgaa
     1381 agtgaaagat gcggaagatc aactgggtgc gcgcgtgggc tatattgaac tggatctgaa
     1441 cagcggcaaa attctggaat cttttcgtcc ggaagaacgt tttccgatga tgagcacctt
     1501 taaagtgctg ctgtgcggtg cggttctgag ccgtgtggat gcgggccagg aacaactggg
     1561 ccgtcgtatt cattatagcc agaacgatct ggtggaatat agcccggtga ccgaaaaaca
     1621 tctgaccgat ggcatgaccg tgcgtgaact gtgcagcgcg gcgattacca tgagcgataa
     1681 caccgcggcg aacctgctgc tgacgaccat tggcggtccg aaagaactga ccgcgtttct
     1741 gcataacatg ggcgatcatg tgacccgtct ggatcgttgg gaaccggaac tgaacgaagc
     1801 gattccgaac gatgaacgtg ataccaccat gccggcagca atggcgacca ccctgcgtaa
     1861 actgctgacg ggtgagctgc tgaccctggc aagccgccag caactgattg attggatgga
     1921 agcggataaa gtggcgggtc cgctgctgcg tagcgcgctg ccggctggct ggtttattgc
     1981 ggataaaagc ggtgcgggcg aacgtggcag ccgtggcatt attgcggcgc tgggcccgga
     2041 tggtaaaccg agccgtattg tggtgattta taccaccggc agccaggcga cgatggatga
     2101 acgtaaccgt cagattgcgg aaattggcgc gagcctgatt aaacattggt aaaccgatac
     2161 aattaaaggc tccttttgga gccttttttt ttggacgacc cttgtccttt tccgctgcat
     2221 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat
     2281 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc
     2341 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt
     2401 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg taggccggcc
     2461 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2521 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2581 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2641 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2701 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2761 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2821 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2881 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2941 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3001 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3061 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3121 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3181 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3241 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3301 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3361 ttaatgtgag ttagctcact cattaggcag gcgcgcccag ctgtctaggg cggcggattt
     3421 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
     3481 gactgagcct ttcgttttat ttgatgcct
//
