LOCUS       sequence-248375-        3254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part O- ORF; TetR; transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-tetR_O
FEATURES             Location/Qualifiers
     source          1..3254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             86..706
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(156..175)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    724..744
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      778..872
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        945..1036
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1037..1105,1106..1897)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1901..1940
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2087..2195
                     /note="incP origin of transfer"
     rep_origin      2231..2819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2720..2739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2973..2990
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3107..3128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3162..3248)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tagcctccag attagataaa agtaaagtga ttaacagcgc
      121 attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa
      181 gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga
      241 cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga
      301 aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca
      361 tcgtgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct
      421 cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc
      481 actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt
      541 cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc
      601 tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat
      661 catatgcgga ttagaaaaac aacttaaatg tgaaagtggt tcgtgagacc tttcatcgcg
      721 acctactagt agcggccgct gcagggagtt gtcttcgaag acttcgctct agtcttggac
      781 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg
      841 gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat tacgatttaa
      901 attagtagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt tatttttcta
      961 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1021 ttgaaaaagg aagagtatga gcattcagca ttttcgtgtg gcgctgattc cgttttttgc
     1081 ggcgttttgc ctgccggtgt ttgcgcatcc ggaaaccctg gtgaaagtga aagatgcgga
     1141 agatcaactg ggtgcgcgcg tgggctatat tgaactggat ctgaacagcg gcaaaattct
     1201 ggaatctttt cgtccggaag aacgttttcc gatgatgagc acctttaaag tgctgctgtg
     1261 cggtgcggtt ctgagccgtg tggatgcggg ccaggaacaa ctgggccgtc gtattcatta
     1321 tagccagaac gatctggtgg aatatagccc ggtgaccgaa aaacatctga ccgatggcat
     1381 gaccgtgcgt gaactgtgca gcgcggcgat taccatgagc gataacaccg cggcgaacct
     1441 gctgctgacg accattggcg gtccgaaaga actgaccgcg tttctgcata acatgggcga
     1501 tcatgtgacc cgtctggatc gttgggaacc ggaactgaac gaagcgattc cgaacgatga
     1561 acgtgatacc accatgccgg cagcaatggc gaccaccctg cgtaaactgc tgacgggtga
     1621 gctgctgacc ctggcaagcc gccagcaact gattgattgg atggaagcgg ataaagtggc
     1681 gggtccgctg ctgcgtagcg cgctgccggc tggctggttt attgcggata aaagcggtgc
     1741 gggcgaacgt ggcagccgtg gcattattgc ggcgctgggc ccggatggta aaccgagccg
     1801 tattgtggtg atttatacca ccggcagcca ggcgacgatg gatgaacgta accgtcagat
     1861 tgcggaaatt ggcgcgagcc tgattaaaca ttggtaaacc gatacaatta aaggctcctt
     1921 ttggagcctt tttttttgga cgacccttgt ccttttccgc tgcataaccc tgcttcgggg
     1981 tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc
     2041 aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg
     2101 aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg
     2161 tgctcaacgg gaatcctgct ctgcgaggct ggccgtaggc cggccccgta gaaaagatca
     2221 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2281 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2341 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2401 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2461 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2521 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2581 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2641 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2701 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2761 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2821 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2881 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2941 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3001 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     3061 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     3121 tcactcatta ggcaggcgcg cccagctgtc tagggcggcg gatttgtcct actcaggaga
     3181 gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt
     3241 tttatttgat gcct
//
