LOCUS       sequence-248396-        3071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part O- ORF; merR; transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-merR_O
FEATURES             Location/Qualifiers
     source          1..3071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    541..561
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      595..689
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        762..853
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(854..922,923..1714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1718..1757
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1904..2012
                     /note="incP origin of transfer"
     rep_origin      2048..2636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2537..2556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2790..2807
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2924..2945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2979..3065)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tagccgaaaa taatttggaa aacctgacca ttggcgtttt
      121 tgccaaggcg gccggggtca acgtggagac aatccgcttc tatcagcgca agggcctgtt
      181 gcgggaaccg gacaagcctt acggcagcat ccgccgctat ggggaggcgg acgtggttcg
      241 ggtgaaattc gtgaaatcgg cacagcggct ggggttcagt ctggacgaga ttgccgagct
      301 gttgcggctc gacgatggca cccactgcga ggaggccagc agcctggccg aacacaagct
      361 caaggacgtg cgcgagaaga tggccgactt ggcgcgcatg gaaaccgtgc tgtctgaact
      421 cgtgtgcgcc tgccatgcac gaaaggggaa tgtttcctgc ccgttgatcg cgtcactaca
      481 gggcgaagca ggcctggcaa ggtcagctat gcctggttcg tgagaccttt catcgcgacc
      541 tactagtagc ggccgctgca gggagttgtc ttcgaagact tcgctctagt cttggactcc
      601 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
      661 gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac gatttaaatt
      721 agtagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat ttttctaaat
      781 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      841 aaaaaggaag agtatgagca ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc
      901 gttttgcctg ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag atgcggaaga
      961 tcaactgggt gcgcgcgtgg gctatattga actggatctg aacagcggca aaattctgga
     1021 atcttttcgt ccggaagaac gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg
     1081 tgcggttctg agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta ttcattatag
     1141 ccagaacgat ctggtggaat atagcccggt gaccgaaaaa catctgaccg atggcatgac
     1201 cgtgcgtgaa ctgtgcagcg cggcgattac catgagcgat aacaccgcgg cgaacctgct
     1261 gctgacgacc attggcggtc cgaaagaact gaccgcgttt ctgcataaca tgggcgatca
     1321 tgtgacccgt ctggatcgtt gggaaccgga actgaacgaa gcgattccga acgatgaacg
     1381 tgataccacc atgccggcag caatggcgac caccctgcgt aaactgctga cgggtgagct
     1441 gctgaccctg gcaagccgcc agcaactgat tgattggatg gaagcggata aagtggcggg
     1501 tccgctgctg cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa gcggtgcggg
     1561 cgaacgtggc agccgtggca ttattgcggc gctgggcccg gatggtaaac cgagccgtat
     1621 tgtggtgatt tataccaccg gcagccaggc gacgatggat gaacgtaacc gtcagattgc
     1681 ggaaattggc gcgagcctga ttaaacattg gtaaaccgat acaattaaag gctccttttg
     1741 gagccttttt ttttggacga cccttgtcct tttccgctgc ataaccctgc ttcggggtca
     1801 ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa
     1861 gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag
     1921 taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc
     1981 tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg ccccgtagaa aagatcaaag
     2041 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2101 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2161 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2221 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2281 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2341 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2401 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2461 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2521 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2581 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2641 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2701 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2761 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     2821 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     2881 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     2941 ctcattaggc aggcgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg
     3001 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
     3061 atttgatgcc t
//
