LOCUS       sequence-248399-        3041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part O- ORF; cueR; transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-cueR_O
FEATURES             Location/Qualifiers
     source          1..3041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    511..531
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      565..659
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        732..823
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(824..892,893..1684)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1688..1727
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1874..1982
                     /note="incP origin of transfer"
     rep_origin      2018..2606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2507..2526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2760..2777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2894..2915
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2949..3035)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tagccaacat cagggatgta gcaaaaatta ccggcctgac
      121 cagcaaagcc attcgcttct atgaagagaa ggggctggtg acgccgccga tgcgcagcga
      181 aaacggttat cgcacctaca cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc
      241 acggcaggtt ggctttaacc tggaagagag cggcgagctg gtgaatctgt ttaacgaccc
      301 gcagcggcac agcgccgacg tcaaacggcg cacgctggag aaggtggcgg agatcgaacg
      361 acacattgag gagctgcaat ccatgcgcga ccagctgctg gcactggcga atgcctgccc
      421 tggggatgac agcgccgact gcccgattat cgaaaatctc tccggctgct gtcatcatcg
      481 ggcaggttcg tgagaccttt catcgcgacc tactagtagc ggccgctgca gggagttgtc
      541 ttcgaagact tcgctctagt cttggactcc tgttgataga tccagtaatg acctcagaac
      601 tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc
      661 caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg ggcttttttt
      721 taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      781 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagca ttcagcattt
      841 tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg ccggtgtttg cgcatccgga
      901 aaccctggtg aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg gctatattga
      961 actggatctg aacagcggca aaattctgga atcttttcgt ccggaagaac gttttccgat
     1021 gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg atgcgggcca
     1081 ggaacaactg ggccgtcgta ttcattatag ccagaacgat ctggtggaat atagcccggt
     1141 gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg cggcgattac
     1201 catgagcgat aacaccgcgg cgaacctgct gctgacgacc attggcggtc cgaaagaact
     1261 gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt gggaaccgga
     1321 actgaacgaa gcgattccga acgatgaacg tgataccacc atgccggcag caatggcgac
     1381 caccctgcgt aaactgctga cgggtgagct gctgaccctg gcaagccgcc agcaactgat
     1441 tgattggatg gaagcggata aagtggcggg tccgctgctg cgtagcgcgc tgccggctgg
     1501 ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc agccgtggca ttattgcggc
     1561 gctgggcccg gatggtaaac cgagccgtat tgtggtgatt tataccaccg gcagccaggc
     1621 gacgatggat gaacgtaacc gtcagattgc ggaaattggc gcgagcctga ttaaacattg
     1681 gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga cccttgtcct
     1741 tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct
     1801 ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc
     1861 aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct
     1921 tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc
     1981 cgtaggccgg ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2041 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2101 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2161 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2221 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2281 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2341 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2401 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2461 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2521 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2581 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2641 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2701 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2761 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     2821 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2881 agcgcaacgc aattaatgtg agttagctca ctcattaggc aggcgcgccc agctgtctag
     2941 ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
     3001 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc t
//
