LOCUS       sequence-24840-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Bissonnette SA, Glazier CM, Stewart MQ, Brown GE, Ellson CD, Yaffe 
            MB
  TITLE     Phosphatidylinositol 3-phosphate-dependent and -independent 
            functions of p40phox in activation of the neutrophil NADPH oxidase.
  JOURNAL   J Biol Chem. 2008 Jan 25. 283(4):2108-19.
  PUBMED    18029359
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jan 25. 283(4):2108-19."
COMMENT     Sequence Label: p40 R58Q/W207R
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             63..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..107
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             111..143
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 ttgcgtaatt ccctctgaat attttgttta ctttaagaag gagatatacc atgggcagca
       61 gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat atggctagca
      121 tgactggtgg acagcaaatg ggtcgcggat ccgaattcat ggctgtggcc cagcagctgc
      181 gggccgagag tgactttgaa cagcttccgg atgatgttgc catctcggcc aacattgctg
      241 acatcgagga gaagagaggc ttcaccagcc actttgtttt cgtcatcgag gtgaagacaa
      301 aaggaggatc caagtacctc atctaccgcc agtaccgcca gttccatgct ttgcagagca
      361 agctggagga gcgcttcggg ccagacagca agagcagtgc cctggcctgt accctgccca
      421 cactcccagc caaagtctac gtgggtgtga aacaggagat cgccgagatg cggatacctg
      481 ccctcaacgc ctacatgaag agcctgctca gcctgccggt ctgggtgctg atggatgagg
      541 acgtccggat cttcttttac cagtcgccct atgactcaga gcaggtgccc caggcactcc
      601 gccggctccg cccgcgcacc cggaaagtca agagcgtgtc cccacagggc aacagcgttg
      661 accgcatggc agctccgaga gcagaggctc tatttgactt cactggaaac agcaaactgg
      721 agctgaattt caaagctgga gatgtgatct tcctcctcag ttcggatcaa caana
//
