LOCUS       sequence-248400-        2909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part O- ORF; lacZ alpha-peptide; blue/white screening. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-lacZ_O
FEATURES             Location/Qualifiers
     source          1..2909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    379..399
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      433..527
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        600..691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(692..760,761..1552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1556..1595
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1742..1850
                     /note="incP origin of transfer"
     rep_origin      1886..2474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2375..2394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2628..2645
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2762..2783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2817..2903)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tagccggtga cccgaaactg ctgctggaag ttccgcacgc
      121 tatctcttct tctccgggta actctctggc tgttgttctg caacgtcgtg actgggaaaa
      181 cccgggtgtt acccagctga accgtctggc tgctcacccg ccgttcgctt cttggcgtaa
      241 ctctgaagaa gctcgtaccg accgtccgtc ccagcagctg cgttctctga tccgtctgct
      301 gaccaaaccg gaacgtaaac tgtcttggct gctgccgccg ctgtctaaca acggttcgtg
      361 agacctttca tcgcgaccta ctagtagcgg ccgctgcagg gagttgtctt cgaagacttc
      421 gctctagtct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
      481 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca ggggtcccca
      541 ataattacga tttaaattag tagcccgcct aatgagcggg ctttttttta attcccctat
      601 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      661 aatgcttcaa taatattgaa aaaggaagag tatgagcatt cagcattttc gtgtggcgct
      721 gattccgttt tttgcggcgt tttgcctgcc ggtgtttgcg catccggaaa ccctggtgaa
      781 agtgaaagat gcggaagatc aactgggtgc gcgcgtgggc tatattgaac tggatctgaa
      841 cagcggcaaa attctggaat cttttcgtcc ggaagaacgt tttccgatga tgagcacctt
      901 taaagtgctg ctgtgcggtg cggttctgag ccgtgtggat gcgggccagg aacaactggg
      961 ccgtcgtatt cattatagcc agaacgatct ggtggaatat agcccggtga ccgaaaaaca
     1021 tctgaccgat ggcatgaccg tgcgtgaact gtgcagcgcg gcgattacca tgagcgataa
     1081 caccgcggcg aacctgctgc tgacgaccat tggcggtccg aaagaactga ccgcgtttct
     1141 gcataacatg ggcgatcatg tgacccgtct ggatcgttgg gaaccggaac tgaacgaagc
     1201 gattccgaac gatgaacgtg ataccaccat gccggcagca atggcgacca ccctgcgtaa
     1261 actgctgacg ggtgagctgc tgaccctggc aagccgccag caactgattg attggatgga
     1321 agcggataaa gtggcgggtc cgctgctgcg tagcgcgctg ccggctggct ggtttattgc
     1381 ggataaaagc ggtgcgggcg aacgtggcag ccgtggcatt attgcggcgc tgggcccgga
     1441 tggtaaaccg agccgtattg tggtgattta taccaccggc agccaggcga cgatggatga
     1501 acgtaaccgt cagattgcgg aaattggcgc gagcctgatt aaacattggt aaaccgatac
     1561 aattaaaggc tccttttgga gccttttttt ttggacgacc cttgtccttt tccgctgcat
     1621 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat
     1681 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc
     1741 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt
     1801 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg taggccggcc
     1861 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1921 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1981 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2041 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2101 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2161 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2221 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2281 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2341 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2401 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2461 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2521 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2581 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2641 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     2701 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     2761 ttaatgtgag ttagctcact cattaggcag gcgcgcccag ctgtctaggg cggcggattt
     2821 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
     2881 gactgagcct ttcgttttat ttgatgcct
//
