LOCUS       sequence-248403-        3347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part O- ORF; cI; transcription factor from lambda phage; 
            thermosensitive..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-cI-ts_O
FEATURES             Location/Qualifiers
     source          1..3347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             86..799
                     /codon_start=1
                     /gene="cIts"
                     /product="temperature-sensitive variant of the phage lambda
                     repressor"
                     /label=lambda repressor (ts)
                     /note="thermosensitivity is conferred by the A67T mutation"
                     /translation="STKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQS
                     GVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEYP
                     VFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDG
                     MLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVV
                     GKVIASQWPEETFGS"
     misc_feature    817..837
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      871..965
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1038..1129
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1130..1198,1199..1990)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1994..2033
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2180..2288
                     /note="incP origin of transfer"
     rep_origin      2324..2912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2813..2832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3066..3083
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3200..3221
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3255..3341)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tagccagcac aaaaaagaaa ccattaacac aagagcagct
      121 tgaggacgca cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc
      181 ccaggaatct gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa
      241 tggcatcaat gcattaaatg cttataacgc cgcattgctt acaaaaattc tcaaagttag
      301 cgttgaagaa tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag
      361 tatgcagccg tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg
      421 gatgttctca cctaagctta gaacctttac caaaggtgat gcggagagat gggtaagcac
      481 aaccaaaaaa gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc
      541 accaacaggc tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca
      601 ggctgttgag ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa
      661 gaaactgatc agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat
      721 gatcccatgc aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga
      781 agagactttt ggttcgtgag acctttcatc gcgacctact agtagcggcc gctgcaggga
      841 gttgtcttcg aagacttcgc tctagtcttg gactcctgtt gatagatcca gtaatgacct
      901 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg
      961 agaatccagg ggtccccaat aattacgatt taaattagta gcccgcctaa tgagcgggct
     1021 tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1081 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagcattca
     1141 gcattttcgt gtggcgctga ttccgttttt tgcggcgttt tgcctgccgg tgtttgcgca
     1201 tccggaaacc ctggtgaaag tgaaagatgc ggaagatcaa ctgggtgcgc gcgtgggcta
     1261 tattgaactg gatctgaaca gcggcaaaat tctggaatct tttcgtccgg aagaacgttt
     1321 tccgatgatg agcaccttta aagtgctgct gtgcggtgcg gttctgagcc gtgtggatgc
     1381 gggccaggaa caactgggcc gtcgtattca ttatagccag aacgatctgg tggaatatag
     1441 cccggtgacc gaaaaacatc tgaccgatgg catgaccgtg cgtgaactgt gcagcgcggc
     1501 gattaccatg agcgataaca ccgcggcgaa cctgctgctg acgaccattg gcggtccgaa
     1561 agaactgacc gcgtttctgc ataacatggg cgatcatgtg acccgtctgg atcgttggga
     1621 accggaactg aacgaagcga ttccgaacga tgaacgtgat accaccatgc cggcagcaat
     1681 ggcgaccacc ctgcgtaaac tgctgacggg tgagctgctg accctggcaa gccgccagca
     1741 actgattgat tggatggaag cggataaagt ggcgggtccg ctgctgcgta gcgcgctgcc
     1801 ggctggctgg tttattgcgg ataaaagcgg tgcgggcgaa cgtggcagcc gtggcattat
     1861 tgcggcgctg ggcccggatg gtaaaccgag ccgtattgtg gtgatttata ccaccggcag
     1921 ccaggcgacg atggatgaac gtaaccgtca gattgcggaa attggcgcga gcctgattaa
     1981 acattggtaa accgatacaa ttaaaggctc cttttggagc cttttttttt ggacgaccct
     2041 tgtccttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc
     2101 catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt
     2161 gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt
     2221 ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag
     2281 gctggccgta ggccggcccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2341 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2401 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2461 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2521 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2581 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2641 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2701 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2761 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2821 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2881 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2941 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3001 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3061 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3121 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     3181 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaggc gcgcccagct
     3241 gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     3301 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcct
//
