LOCUS       sequence-248405-        3623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part NOC- Complete ORF; PheS; counter-selection marker; mutant
            phenylalanine tRNA synthetase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-pheS_NOC
FEATURES             Location/Qualifiers
     source          1..3623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    1093..1113
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1147..1241
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1314..1405
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1406..1474,1475..2266)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2270..2309
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2456..2564
                     /note="incP origin of transfer"
     rep_origin      2600..3188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3089..3108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3342..3359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3476..3497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3531..3617)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc taatgtcaca tctcgcagaa ctggttgcca gtgcgaaggc
      121 ggccattagc caggcgtcag atgttgccgc gttagataat gtgcgcgtcg aatatttggg
      181 taaaaaaggg cacttaaccc ttcagatgac gaccctgcgt gagctgccgc cagaagagcg
      241 tccggcagct ggtgcggtta tcaacgaagc gaaagagcag gttcagcagg cgctgaatgc
      301 gcgtaaagcg gaactggaaa gcgctgcact gaatgcgcgt ctggcggcgg aaacgattga
      361 tgtctctctg ccaggtcgtc gcattgaaaa cggcggtctg catccggtta cccgtaccat
      421 cgaccgtatc gaaagtttct tcggtgagct tggctttacc gtggcaaccg ggccggaaat
      481 cgaagatgat tatcataact tcgatgctct gaacattcct ggtcaccacc cggcgcgcgc
      541 tgaccacgac actttctggt ttgacactac ccgcctgctg cgtacccaga cctctggcgt
      601 acagatccgc accatgaaag cccagcagcc accgattcgt atcattgcgc ctggccgtgt
      661 ttatcgtaac gactacgacc agactcacac gccgatgttc catcagatgg aaggtctgat
      721 tgttgatacc aacatcagct ttaccaacct gaaaggcacg ctgcacgact tcctgcgtaa
      781 cttctttgag gaagatttgc agattcgctt ccgtccttcc tacttcccgt ttaccgaacc
      841 ttctgccgaa gtggacgtca tgggtaaaaa cggtaaatgg ctggaagtgc tgggctgcgg
      901 gatggtgcat ccgaacgtgt tgcgtaacgt tggcatcgac ccggaagttt actctggttt
      961 cggcttcggg atggggatgg agcgtctgac tatgttgcgt tacggcgtca ccgacctgcg
     1021 ttcattcttc gaaaacgatc tgcgtttcct caaacagttt aaataagggc tttgagacct
     1081 ttcatcgcga cctactagta gcggccgctg cagggagttg tcttcgaaga cttcgctcta
     1141 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     1201 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
     1261 acgatttaaa ttagtagccc gcctaatgag cgggcttttt tttaattccc ctatttgttt
     1321 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1381 tcaataatat tgaaaaagga agagtatgag cattcagcat tttcgtgtgg cgctgattcc
     1441 gttttttgcg gcgttttgcc tgccggtgtt tgcgcatccg gaaaccctgg tgaaagtgaa
     1501 agatgcggaa gatcaactgg gtgcgcgcgt gggctatatt gaactggatc tgaacagcgg
     1561 caaaattctg gaatcttttc gtccggaaga acgttttccg atgatgagca cctttaaagt
     1621 gctgctgtgc ggtgcggttc tgagccgtgt ggatgcgggc caggaacaac tgggccgtcg
     1681 tattcattat agccagaacg atctggtgga atatagcccg gtgaccgaaa aacatctgac
     1741 cgatggcatg accgtgcgtg aactgtgcag cgcggcgatt accatgagcg ataacaccgc
     1801 ggcgaacctg ctgctgacga ccattggcgg tccgaaagaa ctgaccgcgt ttctgcataa
     1861 catgggcgat catgtgaccc gtctggatcg ttgggaaccg gaactgaacg aagcgattcc
     1921 gaacgatgaa cgtgatacca ccatgccggc agcaatggcg accaccctgc gtaaactgct
     1981 gacgggtgag ctgctgaccc tggcaagccg ccagcaactg attgattgga tggaagcgga
     2041 taaagtggcg ggtccgctgc tgcgtagcgc gctgccggct ggctggttta ttgcggataa
     2101 aagcggtgcg ggcgaacgtg gcagccgtgg cattattgcg gcgctgggcc cggatggtaa
     2161 accgagccgt attgtggtga tttataccac cggcagccag gcgacgatgg atgaacgtaa
     2221 ccgtcagatt gcggaaattg gcgcgagcct gattaaacat tggtaaaccg atacaattaa
     2281 aggctccttt tggagccttt ttttttggac gacccttgtc cttttccgct gcataaccct
     2341 gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag
     2401 gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca
     2461 ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc
     2521 acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc ggccccgtag
     2581 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2641 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2701 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2761 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2821 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2881 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2941 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3001 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3061 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3121 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3181 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3241 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3301 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3361 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     3421 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     3481 tgagttagct cactcattag gcaggcgcgc ccagctgtct agggcggcgg atttgtccta
     3541 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga
     3601 gcctttcgtt ttatttgatg cct
//
