LOCUS       sequence-248424-       10463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Expression Vektor with CSF2RB wildtype cDNA and IRES 
            eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Labuhn M, Perkins K, Matzk S, Varghese L, Garnett C, Papaemmanuil E,
            Metzner M, Kennedy A, Amstislavskiy V, Risch T, Bhayadia R, 
            Samulowski D, Hernandez DC, Stoilova B, Iotchkova V, Oppermann U, 
            Scheer C, Yoshida K, Schwarzer A, Taub JW, Crispino JD, Weiss MJ, 
            Hayashi Y, Taga T, Ito E, Ogawa S, Reinhardt D, Yaspo ML, Campbell 
            PJ, Roberts I, Constantinescu SN, Vyas P, Heckl D, Klusmann JH
  TITLE     Mechanisms of Progression of Myeloid Preleukemia to Transformed 
            Myeloid Leukemia in Children with Down Syndrome.
  JOURNAL   Cancer Cell. 2019 Aug 12;36(2):123-138.e10. doi: 
            10.1016/j.ccell.2019.06.007. Epub 2019 Jul 11.
  PUBMED    31303423
REFERENCE   2  (bases 1 to 10463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2019.06.007"; journalName: "Cancer Cell"; date: 
            "2019-08-12- 12"; volume: "36"; issue: "2"; pages: "123-138.e10"
COMMENT     Sequence Label: LeGO-iG-CSF2RB
FEATURES             Location/Qualifiers
     source          1..10463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2614..2630
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2672..2705)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2776..3182
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5500..5511
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    join(5910..6484,6485..6487,6488..6496)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6077..6094)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6304..6323
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6497..6499,6500..6502,6503..7216)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6497..6499,6500..6502,6503..7213)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6542..6563)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6803..6822)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7150..7171
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7263..7296)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7352..7940
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7405..7425)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7823..7834)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7943..7959)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8469..8649
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8711..9299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8791..8810)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9470..10261,10262..10330))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10093..10112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10331..10435)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgccgaggtc gacggtatcg ataagctcgc
     2641 ttcacgagat tccagcaggt cgagggacct aataacttcg tatagcatac attatacgaa
     2701 gttatattaa gggttccaag cttaagcggc cggccgctga aagaccccac ctgtaggttt
     2761 ggcaagctag ctgcagtaac gccattttgc aaggcatgga aaaataccaa accaagaata
     2821 gagaagttca gatcaagggc gggtacatga aaatagctaa cgttgggcca aacaggatat
     2881 ctgcggtgag cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg
     2941 gccccggccc gaggccaaga acagatggtc cccagatatg gcccaaccct cagcagtttc
     3001 ttaagaccca tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat
     3061 ttgaattaac caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc
     3121 tataaaagag ctcacaaccc ctcactcggc gcgccagtcc tccgattgac tgagtcgccc
     3181 ggatccatgg tgctggccca gggcctgctg tctatggccc tgctggccct gtgctgggag
     3241 agaagcctcg caggcgccga ggaaaccatc cccctgcaga ccctgcggtg ctacaacgac
     3301 tacaccagcc acatcacctg tagatgggcc gacacccagg acgcccagcg gctggtcaac
     3361 gtgaccctga tcagaagagt gaacgaggac ctgctggaac ccgtgtcctg cgacctgagc
     3421 gacgacatgc cttggagcgc ctgcccccac cctagatgtg tgcctcggag atgcgtgatc
     3481 ccctgccaga gcttcgtggt caccgacgtg gactacttca gcttccagcc cgaccggcct
     3541 ctgggcacca gactgaccgt gacactgacc cagcacgtgc agccccccga gcccagggat
     3601 ctccagatct ctaccgacca ggaccacttc ctgctgactt ggagcgtggc cctcggcagc
     3661 cctcagagcc attggctgag ccctggcgac ctggaattcg aggtggtgta caagcggctg
     3721 caggacagct gggaggacgc tgccatcctg ctgagcaaca ccagccaggc caccctgggc
     3781 cccgagcacc tgatgcctag cagcacctac gtggccagag tgcggaccag actggcccct
     3841 ggcagcaggc tgagcggcag acctagcaag tggtcccccg aagtgtgctg ggacagccag
     3901 ccaggcgacg aggcccagcc ccagaacctg gaatgcttct tcgacggcgc tgccgtgctg
     3961 agctgcagct gggaagtgcg gaaagaggtg gccagcagcg tcagcttcgg cctgttctac
     4021 aagcccagcc ccgacgccgg cgaagaggaa tgcagcccag tgctgagaga gggcctgggc
     4081 tccctgcaca cccggcacca ctgtcagatc cccgtgcccg atcctgccac ccacggccag
     4141 tacatcgtgt ccgtgcagcc cagacgggcc gagaagcaca tcaagagcag cgtgaacatc
     4201 cagatggccc ctcccagcct gaacgtgacc aaggacggag acagctacag cctgagatgg
     4261 gagacaatga agatgcgcta cgagcacatc gaccacacct tcgagatcca gtaccggaag
     4321 gacaccgcca cctggaagga cagcaagacc gagacactgc agaacgccca cagcatggcc
     4381 ctgcccgccc tggaacccag caccagatac tgggccagag tccgcgtgcg gacctccaga
     4441 accggctaca acggcatttg gagcgagtgg tctgaggcca gaagctggga caccgagagc
     4501 gtgctgccta tgtgggtgct ggctctgatc gtgatcttcc tgacaatcgc cgtgctgctg
     4561 gcactgcggt tctgcggcat ctacggctac cggctgcggc ggaagtggga ggaaaagatc
     4621 cccaacccca gcaagagcca cctgttccag aacggctccg ccgagctgtg gcctcctggc
     4681 tccatgagcg ccttcaccag cggctctcca ccccaccagg gcccttgggg cagcagattt
     4741 cctgagctgg aaggcgtgtt ccccgtgggc ttcggcgaca gcgaggtgtc ccctctgacc
     4801 atcgaggacc ccaagcacgt gtgcgacccc cccagcggac ctgatacaac ccctgccgcc
     4861 agcgacctgc ctaccgagca gcctccatct ccccagcctg gacctccagc cgccagccac
     4921 acaccagaga agcaggccag cagcttcgac ttcaacggcc cctacctggg ccctccccac
     4981 agcagaagcc tgcccgatat cctcggccag cccgagcctc ctcaggaagg cggcagccag
     5041 aagtcccctc cacccggcag cctggaatac ctgtgtctgc ctgccggcgg acaggtgcag
     5101 ctggtgcctc tcgctcaggc catgggacct ggacaggctg tggaagtgga aagaaggcct
     5161 agccagggcg ctgccggctc cccatctctg gaatctggcg gaggacctgc ccctcctgcc
     5221 ctgggaccta gagtgggcgg ccaggaccag aaagacagcc ccgtggccat ccccatgagc
     5281 agcggcgata cagaggaccc tggcgtggcc agcggctacg tgtccagcgc cgatctggtg
     5341 ttcaccccca acagcggcgc cagctccgtg tccctggtgc cttctctggg cctgcccagc
     5401 gaccagaccc ctagcctgtg tcctggactg gcctctggac ctcctggcgc ccctggacct
     5461 gtgaagtccg gcttcgaggg ctacgtggaa ctgcccccca tcgagggcag aagcccccgg
     5521 tcccccagaa acaaccctgt gccccctgag gccaagagcc ccgtgctgaa tcccggcgaa
     5581 agacccgccg acgtgtcccc cacaagccct cagccagagg gactgctggt gctgcagcaa
     5641 gtgggagact actgcttcct gcctggcctc ggaccagggc cactgtccct gagaagcaag
     5701 cctagctctc ccggcccagg ccccgagatc aagaacctgg accaggcctt ccaggtcaaa
     5761 aagcctcccg gacaggccgt gccccaggtg cccgtgatcc agctgttcaa ggccctgaag
     5821 cagcaggact acctgagcct gcccccctgg gaagtgaaca agcccggcga agtctgctga
     5881 gcggccgcta cgtaaattcc gccccccccc ccctctccct cccccccccc taacgttact
     5941 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     6001 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     6061 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     6121 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     6181 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     6241 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     6301 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     6361 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     6421 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     6481 taatatggcc acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     6541 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     6601 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     6661 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     6721 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     6781 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     6841 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     6901 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     6961 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     7021 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     7081 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     7141 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     7201 cgagctgtac aagtaaagcg gccggccgcc agcacagtgg tcgaaattcg tcgagggacc
     7261 taataacttc gtatagcata cattatacga agttatacat gtttaagggt tccggttcca
     7321 ctaggtacaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     7381 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     7441 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     7501 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7561 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7621 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     7681 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7741 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     7801 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7861 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7921 cctttgggcc gcctccccgc atcgataccg tcgacctcga tcgagaccta gaaaaacatg
     7981 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     8041 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     8101 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8161 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     8221 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
     8281 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
     8341 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
     8401 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
     8461 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8521 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8581 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8641 tctctagcag catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8701 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8761 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8821 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8881 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8941 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9001 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9061 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9121 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9181 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9241 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9301 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9361 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9421 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9481 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9541 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9601 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9661 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9721 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9781 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9841 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9901 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9961 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10021 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10081 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10141 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10201 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10261 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10321 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10381 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10441 ttccccgaaa agtgccacct gac
//
