LOCUS       sequence-248428-        4076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part T- Terminator;  B0015. Terminator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 13 to 47)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-B0015_T
FEATURES             Location/Qualifiers
     source          1..4076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..47
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    101..122
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      160..231
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      247..274
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    309..329
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             357..377
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             397..1104
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             397..1104
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(421..449)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1044..1064
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1144..1238
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1311..1402
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1403..1471,1472..2263)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2267..2306
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2453..2561
                     /note="incP origin of transfer"
     rep_origin      2597..3185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3086..3105
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3339..3356
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3371..3511
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3437..3456)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3597..3619
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3613..3804)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3984..4070)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctgctttcg accaggcatc aaataaaacg aaaggctcag
      181 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      241 cacactggct caccttcggg tgggcctttc tgcgtttata tgttcgcttg agacctttca
      301 tcgcgaccta ctagtagcgg ccgctgcagg gagtaacttc tgactgagtt gcatacttgt
      361 ttaactttaa gaaggagata tacaatggta gcccgtaaag gagaagaact tttcactgga
      421 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      481 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      541 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
      601 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
      661 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
      721 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      781 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      841 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
      901 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      961 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     1021 cccaacgaaa agagagatca catggtcctt cttgagtttg taacagctgc tgggattaca
     1081 catggcatgg atgaactata caaaggttcg taacttctga ctgagttgca cgctctagtc
     1141 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
     1201 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg
     1261 atttaaatta gtagcccgcc taatgagcgg gctttttttt aattccccta tttgtttatt
     1321 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1381 ataatattga aaaaggaaga gtatgagcat tcagcatttt cgtgtggcgc tgattccgtt
     1441 ttttgcggcg ttttgcctgc cggtgtttgc gcatccggaa accctggtga aagtgaaaga
     1501 tgcggaagat caactgggtg cgcgcgtggg ctatattgaa ctggatctga acagcggcaa
     1561 aattctggaa tcttttcgtc cggaagaacg ttttccgatg atgagcacct ttaaagtgct
     1621 gctgtgcggt gcggttctga gccgtgtgga tgcgggccag gaacaactgg gccgtcgtat
     1681 tcattatagc cagaacgatc tggtggaata tagcccggtg accgaaaaac atctgaccga
     1741 tggcatgacc gtgcgtgaac tgtgcagcgc ggcgattacc atgagcgata acaccgcggc
     1801 gaacctgctg ctgacgacca ttggcggtcc gaaagaactg accgcgtttc tgcataacat
     1861 gggcgatcat gtgacccgtc tggatcgttg ggaaccggaa ctgaacgaag cgattccgaa
     1921 cgatgaacgt gataccacca tgccggcagc aatggcgacc accctgcgta aactgctgac
     1981 gggtgagctg ctgaccctgg caagccgcca gcaactgatt gattggatgg aagcggataa
     2041 agtggcgggt ccgctgctgc gtagcgcgct gccggctggc tggtttattg cggataaaag
     2101 cggtgcgggc gaacgtggca gccgtggcat tattgcggcg ctgggcccgg atggtaaacc
     2161 gagccgtatt gtggtgattt ataccaccgg cagccaggcg acgatggatg aacgtaaccg
     2221 tcagattgcg gaaattggcg cgagcctgat taaacattgg taaaccgata caattaaagg
     2281 ctccttttgg agcctttttt tttggacgac ccttgtcctt ttccgctgca taaccctgct
     2341 tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat
     2401 tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga
     2461 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
     2521 tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc cccgtagaaa
     2581 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2641 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2701 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2761 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2821 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2881 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2941 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3001 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3061 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3121 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3181 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3241 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3301 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3361 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3421 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     3481 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     3541 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga cagtctccgg
     3601 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta
     3661 aagctcatca gcgtggtcgt gcagcgattc acagatgtct gcctgttcat ccgcgtccag
     3721 ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag
     3781 ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg
     3841 ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca
     3901 tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggggcg
     3961 cgcccagctg tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     4021 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcct
//
