LOCUS       sequence-24843-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Bissonnette SA, Glazier CM, Stewart MQ, Brown GE, Ellson CD, Yaffe 
            MB
  TITLE     Phosphatidylinositol 3-phosphate-dependent and -independent 
            functions of p40phox in activation of the neutrophil NADPH oxidase.
  JOURNAL   J Biol Chem. 2008 Jan 25. 283(4):2108-19.
  PUBMED    18029359
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jan 25. 283(4):2108-19."
COMMENT     Sequence Label: p40 R58Q
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..106
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             110..142
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 tgngnaattc cctctgaata ttttgtttac tttaagaagg agatatacca tgggcagcag
       61 ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata tggctagcat
      121 gactggtgga cagcaaatgg gtcgcggatc cgaattcatg gctgtggccc agcagctgcg
      181 ggccgagagt gactttgaac agcttccgga tgatgttgcc atctcggcca acattgctga
      241 catcgaggag aagagaggct tcaccagcca ctttgttttc gtcatcgagg tgaagacaaa
      301 aggaggatcc aagtacctca tctaccgcca gtaccgccag ttccatgctt tgcagagcaa
      361 gctggaggag cgcttcgggc cagacagcaa gagcagtgcc ctggcctgta ccctgcccac
      421 actcccagcc aaagtctacg tgggtgtgaa acaggagatc gccgagatgc ggatacctgc
      481 cctcaacgcc tacatgaaga gcctgctcag cctgccggtc tgggtgctga tggatgagga
      541 cgtccggatc ttcttttacc agtcgcccta tgactcagag caggtgcccc aggcactccg
      601 ccggctccgc ccgcgcaccc ggaaagtcaa gagcgtgtcc ccacagggaa aacagcgttg
      661 accgcatggc agctccgaga tgagaggctc tatttgactt cactggaaac agcaaactgg
      721 agctgaattt caaagctgga gatgtgatct tcctcctcag tcggatcaac aaaagactgg
      781 ctggagggca ctgtccgggg agccacgggc atcttcctct t
//
