LOCUS       sequence-248431-        8370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  YFP fusion of Sec31A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Stephens DJ, Lin-Marq N, Pagano A, Pepperkok R, Paccaud JP
  TITLE     COPI-coated ER-to-Golgi transport complexes segregate from COPII in 
            close proximity to ER exit sites.
  JOURNAL   J Cell Sci. 2000 Jun;113 ( Pt 12):2177-85.
  PUBMED    10825291
REFERENCE   2  (bases 1 to 8370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2000-06"; volume: "113 ( Pt 12)"; pages: "2177-85"
COMMENT     Sequence Label: pEYFP-Sec31A
FEATURES             Location/Qualifiers
     source          1..8370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5098..5219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5135..5154)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5189..5208
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5226..5681)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5363..5384)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5575..5594
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5708..5812
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5814..6171
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5814..6005
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5837..5857)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6022..6157
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6206..7000
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6206..7000
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6260..6279)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6870..6889
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7188..7207)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7232..7279
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7608..8196
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8097..8116
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1021 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt ccggactcag atctcgagct caagcttcga attcgatgaa gttaaaggaa
     1321 gtagatcgta cagccatgca ggcatggagc cctgcccaga atcaccccat ttacctagca
     1381 acaggaacat ctgctcagca attggatgca acatttagta cgaatgcttc ccttgagata
     1441 tttgaattag acctctctga tccatccttg gatatgaaat cttgtgccac attctcctct
     1501 tctcacaggt accacaagtt gatttggggg ccttataaaa tggattccaa aggagatgtc
     1561 tctggagttc tgattgcagg tggtgaaaat ggaaatatta ttctctatga tccttctaaa
     1621 attatagctg gagacaagga agttgtgatt gcccagaatg acaagcatac tggcccagtg
     1681 agagccttgg atgtgaacat tttccagact aatctggtag cttctggtgc taatgaatct
     1741 gaaatctaca tatgggatct aaataatttt gcaaccccaa tgacaccagg agccaaaaca
     1801 cagccgccag aagatatcag ctgcattgca tggaacagac aagttcagca tattttagca
     1861 tcagccagtc ccagtggccg ggccactgta tgggatctta ggaaaaatga gccaatcatc
     1921 aaagtcagtg accatagtaa cagaatgcat tgttctgggt tggcatggca tcctgatgtt
     1981 gctactcaga tggtccttgc ctccgaggat gaccggttac cagtgatcca gatgtgggat
     2041 cttcgatttg cttcctctcc acttcgtgtc ctggaaaacc atgccagggg gattttggca
     2101 attgcttgga gcatggcaga tcctgaattg ttactgagct gtggaaaaga tgctaagatt
     2161 ctctgctcca atccaaacac aggagaggtg ttatatgaac ttcccaccaa cacacagtgg
     2221 tgcttcgata ttcagtggtg tccccgaaat cctgctgtct tatcagctgc ttcgtttgat
     2281 gggcgtatca gtgtttattc tatcatggga ggaagcacag atggtttaag acagaaacaa
     2341 gttgacaagc tttcatcatc ttttgggaat cttgatccct ttggcacagg acagcccctt
     2401 cctccgttac aaattccaca gcagactgct cagcatagta tagtgctgcc tctgaagaag
     2461 ccgcccaagt ggattcgaag gcctgttggt gcttcttttt catttggagg caaactggtt
     2521 acgtttgaga atgtcagaat gccttctcat cagggagctg agcagcagca gcagcagcac
     2581 catgtgttca ttagtcaggt tgtaacagaa aaggagttcc tcagccgatc agaccaactt
     2641 cagcaggctg tgcagtcaca aggatttatc aattattgcc aaaaaaaaat tgatgcttct
     2701 cagactgaat ttgagaaaaa tgtgtggtcc tttttgaagg taaactttga ggatgattct
     2761 cgtggaaaat accttgaact tctaggatac agaaaagaag atctaggaaa gaagattgct
     2821 ttggccttga acaaagtgga tggagccaat gtggctctta aagactctga ccaagtagca
     2881 cagagtgatg gggaggagag ccctgctgct gaagagcagc tcttgggaga gcacattaaa
     2941 gaggaaaaag aagaatctga atttctaccc tcatctggag gaacatttaa tatctctgtc
     3001 agtggggaca ttgatggttt aattactcag gctttgctga cgggcaattt tgagagtgct
     3061 gttgaccttt gtttacatga taaccgcatg gccgatgcca ttatattggc catagcaggt
     3121 ggacaagaac tcttggctcg aacccagaaa aaatacttcg caaaatccca aagcaaaatt
     3181 accaggctca tcactgcagt ggtgatgaag aactggaaag agattgttga gtcttgtgat
     3241 cttaaaaatt ggagagaggc tttagctgca gtattgactt atgcaaagcc ggatgaattt
     3301 tcagcccttt gtgatctttt gggaaccagg cttgaaaatg aaggagatag cctcctgcag
     3361 actcaagcat gtctctgcta tatttgtgcg gggaatgtag agaaattagt tgcatgttgg
     3421 actaaagctc aagatggaag ccaccctttg tcacttcagg atctgattga gaaagttgtc
     3481 atcctgcgaa aagctgtgca actcactcaa gccatggaca ctagtactgt aggagttctc
     3541 ttggctgcga agatgagtca gtatgccaat ttgttggcag ctcagggcag tattgctgca
     3601 gccttggctt ttcttcctga caacaccaac cagccaaata tcatgcagct tcgtgacaga
     3661 ctttgtagag cacaaggaga gcctgtagca ggacatgaat cacctaaaat tccgtacgag
     3721 aaacagcagc tccccaaggg caggcctgga ccagttgctg gccaccacca gatgccaaga
     3781 gttcaaactc aacaatatta tccccatgga gaaaatcctc cacctccggg tttcataatg
     3841 catggaaatg ttaatccaaa tgctgctggt cagcttccca catctccagg tcatatgcac
     3901 acccaggtac caccttatcc acagccacag ccttatcaac cagcccagcc gtatcccttc
     3961 ggaacagggg ggtcagcaat gtatcgacct cagcagcctg ttgctcctcc tacttcaaac
     4021 gcttacccta acacccctta catatcttct gcttcttcct atactgggca gtctcagctg
     4081 tacgcagcac agcaccaggc ctcttcacct acctccagcc ctgctacttc tttccctcct
     4141 cccccttcct ctggagcatc cttccagcat ggcggaccag gagctccacc atcatcttca
     4201 gcttatgcac tgcctcctgg aacaacaggt acactgcctg ctgccagtga gctgcctgcg
     4261 tcccaaagaa caggtcctca gaatggttgg aatgaccctc cagctttgaa cagagtaccc
     4321 aaaaagaaga agatgcctga aaacttcatg cctcctgttc ccatcacatc accaatcatg
     4381 aacccgttgg gtgaccccca gtcacaaatg ctgcagcaac agccttcagc tccagtacca
     4441 ctgtcaagcc agtcttcatt cccacagcca catcttccag gtggccagcc cttccatggc
     4501 gtacagcaac ctcttggtca aacaggcatg ccaccatctt tttcaaagcc caatattgaa
     4561 ggtgccccag gggctcctat tggaaatacc ttccagcatg tgcagtcttt gccaacaaaa
     4621 aaaattacca agaaacctat tccagatgag cacctcattc taaagaccac atttgaggat
     4681 cttattcagc gctgcctttc ttcagcaaca gaccctcaaa ccaagaggaa gctagatgat
     4741 gccagcaaac gtttggagtt tctgtatgat aaacttaggg aacagacact ttcaccaaca
     4801 atcaccagtg gtttacacaa cattgcaagg agcattgaaa ctcgaaacta ctcagaagga
     4861 ttgaccatgc atacccacat agttagcacc agcaacttca gtgagacctc tgctttcatg
     4921 ccagttctca aagttgttct cacccaggcc aataagctgg gtgtctaagg atccaccgga
     4981 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     5041 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     5101 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5161 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     5221 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     5281 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     5341 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     5401 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     5461 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     5521 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     5581 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     5641 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     5701 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5761 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     5821 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     5881 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     5941 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     6001 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6061 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6121 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     6181 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     6241 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     6301 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     6361 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     6421 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     6481 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     6541 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6601 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6661 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6721 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6781 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6841 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6901 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     6961 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     7021 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     7081 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     7141 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     7201 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     7261 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     7321 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     7381 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     7441 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7501 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7561 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7621 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7681 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7741 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7801 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7861 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7921 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7981 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8041 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8101 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8161 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8221 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8281 tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
     8341 attagttcat agcccatata tggagttccg
//
