LOCUS       sequence-248461-        4079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part CT- C-terminus + Terminator; B0015. C-termini STOP + 
            Terminator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 13 to 47)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-B0015_CT
FEATURES             Location/Qualifiers
     source          1..4079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..47
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    101..122
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      163..234
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      250..277
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    312..332
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             360..380
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             400..1107
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             400..1107
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(424..452)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1047..1067
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1147..1241
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1314..1405
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1406..1474,1475..2266)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2270..2309
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2456..2564
                     /note="incP origin of transfer"
     rep_origin      2600..3188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3089..3108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3342..3359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3374..3514
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3440..3459)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3600..3622
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3616..3807)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3987..4073)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctttcgtga tcgaccaggc atcaaataaa acgaaaggct
      181 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      241 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatgttcgc ttgagacctt
      301 tcatcgcgac ctactagtag cggccgctgc agggagtaac ttctgactga gttgcatact
      361 tgtttaactt taagaaggag atatacaatg gtagcccgta aaggagaaga acttttcact
      421 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      481 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      541 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa
      601 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
      661 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
      721 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
      781 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
      841 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
      901 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
      961 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
     1021 gatcccaacg aaaagagaga tcacatggtc cttcttgagt ttgtaacagc tgctgggatt
     1081 acacatggca tggatgaact atacaaaggt tcgtaacttc tgactgagtt gcacgctcta
     1141 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     1201 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
     1261 acgatttaaa ttagtagccc gcctaatgag cgggcttttt tttaattccc ctatttgttt
     1321 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1381 tcaataatat tgaaaaagga agagtatgag cattcagcat tttcgtgtgg cgctgattcc
     1441 gttttttgcg gcgttttgcc tgccggtgtt tgcgcatccg gaaaccctgg tgaaagtgaa
     1501 agatgcggaa gatcaactgg gtgcgcgcgt gggctatatt gaactggatc tgaacagcgg
     1561 caaaattctg gaatcttttc gtccggaaga acgttttccg atgatgagca cctttaaagt
     1621 gctgctgtgc ggtgcggttc tgagccgtgt ggatgcgggc caggaacaac tgggccgtcg
     1681 tattcattat agccagaacg atctggtgga atatagcccg gtgaccgaaa aacatctgac
     1741 cgatggcatg accgtgcgtg aactgtgcag cgcggcgatt accatgagcg ataacaccgc
     1801 ggcgaacctg ctgctgacga ccattggcgg tccgaaagaa ctgaccgcgt ttctgcataa
     1861 catgggcgat catgtgaccc gtctggatcg ttgggaaccg gaactgaacg aagcgattcc
     1921 gaacgatgaa cgtgatacca ccatgccggc agcaatggcg accaccctgc gtaaactgct
     1981 gacgggtgag ctgctgaccc tggcaagccg ccagcaactg attgattgga tggaagcgga
     2041 taaagtggcg ggtccgctgc tgcgtagcgc gctgccggct ggctggttta ttgcggataa
     2101 aagcggtgcg ggcgaacgtg gcagccgtgg cattattgcg gcgctgggcc cggatggtaa
     2161 accgagccgt attgtggtga tttataccac cggcagccag gcgacgatgg atgaacgtaa
     2221 ccgtcagatt gcggaaattg gcgcgagcct gattaaacat tggtaaaccg atacaattaa
     2281 aggctccttt tggagccttt ttttttggac gacccttgtc cttttccgct gcataaccct
     2341 gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag
     2401 gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca
     2461 ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc
     2521 acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc ggccccgtag
     2581 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2641 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2701 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     2761 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2821 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2881 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2941 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3001 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3061 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3121 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3181 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3241 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3301 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3361 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3421 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     3481 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     3541 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgacagtctc
     3601 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
     3661 gtaaagctca tcagcgtggt cgtgcagcga ttcacagatg tctgcctgtt catccgcgtc
     3721 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
     3781 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
     3841 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
     3901 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcgggg
     3961 gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa
     4021 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcct
//
