LOCUS       sequence-248462-        4011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part CT- C-terminus + Terminator; L3S2P21. C-termini STOP + 
            terminator (synthetic).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 13 to 47)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-L3S2P21_CT
FEATURES             Location/Qualifiers
     source          1..4011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..47
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    101..122
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    244..264
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             292..312
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             332..1039
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             332..1039
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(356..384)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     979..999
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1079..1173
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1246..1337
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1338..1406,1407..2198)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2202..2241
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2388..2496
                     /note="incP origin of transfer"
     rep_origin      2532..3120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3021..3040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3274..3291
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3306..3446
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3372..3391)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3532..3554
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3548..3739)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3919..4005)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctttcgtga tcgactcggt accaaattcc agaaaagagg
      181 cctcccgaaa ggggggcctt ttttcgtttt ggtcctgttc gcttgagacc tttcatcgcg
      241 acctactagt agcggccgct gcagggagta acttctgact gagttgcata cttgtttaac
      301 tttaagaagg agatatacaa tggtagcccg taaaggagaa gaacttttca ctggagttgt
      361 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
      421 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
      481 actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc
      541 gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta
      601 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
      661 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
      721 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
      781 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga
      841 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
      901 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
      961 cgaaaagaga gatcacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
     1021 catggatgaa ctatacaaag gttcgtaact tctgactgag ttgcacgctc tagtcttgga
     1081 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     1141 ggttgccgcc gggcgttttt tattggtgag aatccagggg tccccaataa ttacgattta
     1201 aattagtagc ccgcctaatg agcgggcttt tttttaattc ccctatttgt ttatttttct
     1261 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1321 attgaaaaag gaagagtatg agcattcagc attttcgtgt ggcgctgatt ccgttttttg
     1381 cggcgttttg cctgccggtg tttgcgcatc cggaaaccct ggtgaaagtg aaagatgcgg
     1441 aagatcaact gggtgcgcgc gtgggctata ttgaactgga tctgaacagc ggcaaaattc
     1501 tggaatcttt tcgtccggaa gaacgttttc cgatgatgag cacctttaaa gtgctgctgt
     1561 gcggtgcggt tctgagccgt gtggatgcgg gccaggaaca actgggccgt cgtattcatt
     1621 atagccagaa cgatctggtg gaatatagcc cggtgaccga aaaacatctg accgatggca
     1681 tgaccgtgcg tgaactgtgc agcgcggcga ttaccatgag cgataacacc gcggcgaacc
     1741 tgctgctgac gaccattggc ggtccgaaag aactgaccgc gtttctgcat aacatgggcg
     1801 atcatgtgac ccgtctggat cgttgggaac cggaactgaa cgaagcgatt ccgaacgatg
     1861 aacgtgatac caccatgccg gcagcaatgg cgaccaccct gcgtaaactg ctgacgggtg
     1921 agctgctgac cctggcaagc cgccagcaac tgattgattg gatggaagcg gataaagtgg
     1981 cgggtccgct gctgcgtagc gcgctgccgg ctggctggtt tattgcggat aaaagcggtg
     2041 cgggcgaacg tggcagccgt ggcattattg cggcgctggg cccggatggt aaaccgagcc
     2101 gtattgtggt gatttatacc accggcagcc aggcgacgat ggatgaacgt aaccgtcaga
     2161 ttgcggaaat tggcgcgagc ctgattaaac attggtaaac cgatacaatt aaaggctcct
     2221 tttggagcct ttttttttgg acgacccttg tccttttccg ctgcataacc ctgcttcggg
     2281 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc
     2341 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt
     2401 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg
     2461 gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg ccggccccgt agaaaagatc
     2521 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2581 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2641 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2701 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2761 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2821 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2881 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2941 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3001 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3061 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3121 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3181 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3241 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3301 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     3361 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     3421 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     3481 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgacagtc tccgggagct
     3541 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     3601 catcagcgtg gtcgtgcagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     3661 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     3721 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     3781 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     3841 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gggcgcgccc
     3901 agctgtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3961 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc t
//
