LOCUS       sequence-248463-        4002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part CT- C-terminus + Terminator; L3S1P51. C-termini STOP + 
            terminator (synthetic).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 13 to 47)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-L3S1P51_CT
FEATURES             Location/Qualifiers
     source          1..4002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..47
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    101..122
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    235..255
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             283..303
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             323..1030
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             323..1030
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(347..375)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     970..990
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1070..1164
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1237..1328
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1329..1397,1398..2189)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2193..2232
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2379..2487
                     /note="incP origin of transfer"
     rep_origin      2523..3111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3012..3031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3265..3282
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3297..3437
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3363..3382)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3523..3545
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3539..3730)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3910..3996)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctttcgtga tcgaaaaaaa aaaaaaggcc tcccaaatcg
      181 gggggccttt tttattgata acaaaatgtt cgcttgagac ctttcatcgc gacctactag
      241 tagcggccgc tgcagggagt aacttctgac tgagttgcat acttgtttaa ctttaagaag
      301 gagatataca atggtagccc gtaaaggaga agaacttttc actggagttg tcccaattct
      361 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      421 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      481 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
      541 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      601 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
      661 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
      721 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
      781 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
      841 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
      901 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
      961 agatcacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     1021 actatacaaa ggttcgtaac ttctgactga gttgcacgct ctagtcttgg actcctgttg
     1081 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
     1141 cgggcgtttt ttattggtga gaatccaggg gtccccaata attacgattt aaattagtag
     1201 cccgcctaat gagcgggctt ttttttaatt cccctatttg tttatttttc taaatacatt
     1261 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1321 ggaagagtat gagcattcag cattttcgtg tggcgctgat tccgtttttt gcggcgtttt
     1381 gcctgccggt gtttgcgcat ccggaaaccc tggtgaaagt gaaagatgcg gaagatcaac
     1441 tgggtgcgcg cgtgggctat attgaactgg atctgaacag cggcaaaatt ctggaatctt
     1501 ttcgtccgga agaacgtttt ccgatgatga gcacctttaa agtgctgctg tgcggtgcgg
     1561 ttctgagccg tgtggatgcg ggccaggaac aactgggccg tcgtattcat tatagccaga
     1621 acgatctggt ggaatatagc ccggtgaccg aaaaacatct gaccgatggc atgaccgtgc
     1681 gtgaactgtg cagcgcggcg attaccatga gcgataacac cgcggcgaac ctgctgctga
     1741 cgaccattgg cggtccgaaa gaactgaccg cgtttctgca taacatgggc gatcatgtga
     1801 cccgtctgga tcgttgggaa ccggaactga acgaagcgat tccgaacgat gaacgtgata
     1861 ccaccatgcc ggcagcaatg gcgaccaccc tgcgtaaact gctgacgggt gagctgctga
     1921 ccctggcaag ccgccagcaa ctgattgatt ggatggaagc ggataaagtg gcgggtccgc
     1981 tgctgcgtag cgcgctgccg gctggctggt ttattgcgga taaaagcggt gcgggcgaac
     2041 gtggcagccg tggcattatt gcggcgctgg gcccggatgg taaaccgagc cgtattgtgg
     2101 tgatttatac caccggcagc caggcgacga tggatgaacg taaccgtcag attgcggaaa
     2161 ttggcgcgag cctgattaaa cattggtaaa ccgatacaat taaaggctcc ttttggagcc
     2221 tttttttttg gacgaccctt gtccttttcc gctgcataac cctgcttcgg ggtcattata
     2281 gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt
     2341 cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc
     2401 ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac
     2461 gggaatcctg ctctgcgagg ctggccgtag gccggccccg tagaaaagat caaaggatct
     2521 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2581 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2641 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2701 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2761 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2821 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2881 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2941 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3001 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3061 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3121 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3181 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3241 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     3301 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     3361 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     3421 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3481 gtctgctccc ggcatccgct tacagacaag ctgtgacagt ctccgggagc tgcatgtgtc
     3541 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
     3601 ggtcgtgcag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
     3661 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     3721 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
     3781 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
     3841 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggggcgcgcc cagctgtcta
     3901 gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     3961 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ct
//
