LOCUS       sequence-248468-        3476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part Whole TU; Codes for chloramphenicol resistance; including
            promoter and terminator. Broad host range. From Bacillus/E.coli 
            shuttle pSEVA3b61..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-cat_TU
FEATURES             Location/Qualifiers
     source          1..3476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    946..966
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1000..1094
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1167..1258
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1259..1327,1328..2119)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2123..2162
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2309..2417
                     /note="incP origin of transfer"
     rep_origin      2453..3041
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2942..2961
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3195..3212
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3329..3350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3384..3470)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggagggtaa aacaaacggg attgactttt aaaaaaggat
      121 tgattctaat gaagaaagca gacaagtaag cctcctaaat tcactttaga taaaaattta
      181 ggaggcatat caaatgaact ttaataaaat tgatttagac aattggaaga gaaaagagat
      241 atttaatcat tatttgaacc aacaaacgac ttttagtata accacagaaa ttgatattag
      301 tgttttatac cgaaacataa aacaagaagg atataaattt taccctgcat ttattttctt
      361 agtgacaagg gtgataaact caaatacagc ttttagaact ggttacaata gcgacggaga
      421 gttaggttat tgggataagt tagagccact ttatacaatt tttgatggtg tatctaaaac
      481 attctctggt atttggactc ctgtaaagaa tgacttcaaa gagttttatg atttatacct
      541 ttctgatgta gagaaatata atggttcggg gaaattgttt cccaaaacac ctatacctga
      601 aaatgctttt tctctttcta ttattccatg gacttcattt actgggttta acttaaatat
      661 caataataat agtaattacc ttctacccat tattacagca ggaaaattca ttaataaagg
      721 taattcaata tatttaccgc tatctttaca ggtacatcat tctgtttgtg atggttatca
      781 tgcaggattg tttatgaact ctattcagga attgtcagat aggcctaatg actggctttt
      841 ataatatgag ataatgccga ctgtactttt tacagtcggt tttctaatgt cactagggct
      901 cgcctttggg aagtttgaag gcgcttgaga cctttcatcg cgacctacta gtagcggccg
      961 ctgcagggag ttgtcttcga agacttcgct ctagtcttgg actcctgttg atagatccag
     1021 taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
     1081 ttattggtga gaatccaggg gtccccaata attacgattt aaattagtag cccgcctaat
     1141 gagcgggctt ttttttaatt cccctatttg tttatttttc taaatacatt caaatatgta
     1201 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1261 gagcattcag cattttcgtg tggcgctgat tccgtttttt gcggcgtttt gcctgccggt
     1321 gtttgcgcat ccggaaaccc tggtgaaagt gaaagatgcg gaagatcaac tgggtgcgcg
     1381 cgtgggctat attgaactgg atctgaacag cggcaaaatt ctggaatctt ttcgtccgga
     1441 agaacgtttt ccgatgatga gcacctttaa agtgctgctg tgcggtgcgg ttctgagccg
     1501 tgtggatgcg ggccaggaac aactgggccg tcgtattcat tatagccaga acgatctggt
     1561 ggaatatagc ccggtgaccg aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg
     1621 cagcgcggcg attaccatga gcgataacac cgcggcgaac ctgctgctga cgaccattgg
     1681 cggtccgaaa gaactgaccg cgtttctgca taacatgggc gatcatgtga cccgtctgga
     1741 tcgttgggaa ccggaactga acgaagcgat tccgaacgat gaacgtgata ccaccatgcc
     1801 ggcagcaatg gcgaccaccc tgcgtaaact gctgacgggt gagctgctga ccctggcaag
     1861 ccgccagcaa ctgattgatt ggatggaagc ggataaagtg gcgggtccgc tgctgcgtag
     1921 cgcgctgccg gctggctggt ttattgcgga taaaagcggt gcgggcgaac gtggcagccg
     1981 tggcattatt gcggcgctgg gcccggatgg taaaccgagc cgtattgtgg tgatttatac
     2041 caccggcagc caggcgacga tggatgaacg taaccgtcag attgcggaaa ttggcgcgag
     2101 cctgattaaa cattggtaaa ccgatacaat taaaggctcc ttttggagcc tttttttttg
     2161 gacgaccctt gtccttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt
     2221 cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact
     2281 ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga
     2341 gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg
     2401 ctctgcgagg ctggccgtag gccggccccg tagaaaagat caaaggatct tcttgagatc
     2461 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2521 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2581 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2641 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2701 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2761 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2821 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2881 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2941 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3001 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3061 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3121 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3181 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3241 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     3301 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcaggcg
     3361 cgcccagctg tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     3421 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcct
//
