LOCUS       sequence-248469-        4647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 vector. sfGFP reporter. Origin RK2 (broad-host-range). 
            CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 83 to 117)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP42x-2A(sfGFP)
FEATURES             Location/Qualifiers
     source          1..4647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        83..117
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             122..142
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..869
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    922..942
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      976..1070
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1306..2097
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            2243..2351
                     /note="incP origin of transfer"
     CDS             complement(2412..3560)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     terminator      complement(4555..4641)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctctgga gtgagacggg agttgacggc tagctcagtc ctaggtacag tgctagcgta
      121 cttgtttaac tttaagaagg agatatacaa tggtagcccg taaaggcgaa gagctgttca
      181 ctggtgtcgt ccctattctg gtggaactgg atggtgatgt caacggtcat aagttttccg
      241 tgcgtggcga gggtgaaggt gacgcaacta atggtaaact gacgctgaag ttcatctgta
      301 ctactggtaa actgccggta ccttggccga ctctggtaac gacgctgact tatggtgttc
      361 agtgctttgc tcgttatccg gaccatatga agcagcatga cttcttcaag tccgccatgc
      421 cggaaggcta tgtgcaggaa cgcacgattt cctttaagga tgacggcacg tacaaaacgc
      481 gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg cattgagctg aaaggcattg
      541 actttaaaga agatggcaat atcctgggcc ataagctgga atacaatttt aacagccaca
      601 atgtttacat caccgccgat aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc
      661 acaacgtgga ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac actccaatcg
      721 gtgatggtcc tgttctgctg ccagacaatc actatctgag cacgcaaagc gttctgtcta
      781 aagatccgaa cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc gcagcgggca
      841 tcacgcatgg tatggatgaa ctgtacaaag gttcgtaact tctgactgag ttgcacgctc
      901 gtctctcgct aatgtgagac ctactagtag cggccgctgc agggagttgt cttcgaagac
      961 ttcgctctag tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
     1021 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc
     1081 ccaataatta cgatttacgt atttaaatga accttgaccg aacgcagcgg tggtaacggc
     1141 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     1201 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     1261 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg
     1321 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     1381 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     1441 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     1501 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     1561 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     1621 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     1681 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     1741 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     1801 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggtga tgagcgaaat
     1861 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     1921 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     1981 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     2041 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataagac
     2101 aattgtcctt ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat
     2161 atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt
     2221 ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt
     2281 gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc
     2341 gaggctggcc gtaggccggc cgcgatgcag gtggctgctg aacccccagc cggaactgac
     2401 cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc
     2461 aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg
     2521 cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc
     2581 cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc
     2641 tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc
     2701 aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc
     2761 gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg
     2821 cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc
     2881 tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac
     2941 tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag
     3001 gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg
     3061 attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc
     3121 atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc
     3181 ttcgtgtgtt tcagcaacgc ggcctgcttg gcttcgctga cctgttttgc caggtcctcg
     3241 ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc
     3301 gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg
     3361 ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg
     3421 actatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg
     3481 atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc
     3541 cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccggaatt aattccccgg
     3601 atcgatccgt cgatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc
     3661 agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt
     3721 cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta
     3781 cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca
     3841 tccggggtca gcaccgtttc tgcggactgg ctttctacgt ggctgccatt tttggggtga
     3901 ggccgttcgc ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc
     3961 gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg
     4021 cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt
     4081 tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac
     4141 agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc
     4201 aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca
     4261 cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg
     4321 ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct
     4381 gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc cggcggccct acatggctct
     4441 gctgtagtga gtgggttgcg ctccggcagc ggtcctgatc ccccgcagaa aaaaaggatc
     4501 tcaagaagat cctttgatct tttctacggc gcgcccagct gtctagggcg gcggatttgt
     4561 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
     4621 ctgagccttt cgttttattt gatgcct
//
