LOCUS       sequence-24851-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Bissonnette SA, Glazier CM, Stewart MQ, Brown GE, Ellson CD, Yaffe 
            MB
  TITLE     Phosphatidylinositol 3-phosphate-dependent and -independent 
            functions of p40phox in activation of the neutrophil NADPH oxidase.
  JOURNAL   J Biol Chem. 2008 Jan 25. 283(4):2108-19.
  PUBMED    18029359
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jan 25. 283(4):2108-19."
COMMENT     Sequence Label: p40deltaPX (w/out aa1-135)
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..105
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             109..141
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 gnggnattcc ntctnaatat tttgtttact ttaagaagga gatataccat gggcagcagc
       61 catcatcatc atcatcacag cagcggcctg gtgccgcgcg gcagccatat ggctagcatg
      121 actggtggac agcaaatggg tcgcggatcc gaattccagt cgccctatga ctcagagcag
      181 gtgccccagg cactccgccg gctccgcccg cgcacccgga aagtcaagag cgtgtcccca
      241 cagggcaaca gcgttgaccg catggcagct ccgagagcag aggctctatt tgacttcact
      301 ggaaacagca aactggagct gaatttcaaa gctggagatg tgatcttcct cctcagtcgg
      361 atcaacaaag actggctgga gggcactgtc cggggagcca cgggcatctt ccctctctcc
      421 ttcgtgaaga tcctcaaaga cttccctgag gaggacgacc ccaccaactg gctgcgttgc
      481 tactactacg aagacaccat cagcaccatc aaggacatcg cggtggagga agatctcagc
      541 agcactcccc tattgaaaga cctgctggag ctcacaaggc gggagttcca gagagaggac
      601 atagctctga attaccggga cgctgagggg gatctgtcnn gtnnnctgtc ggagaaggac
      661 gnancnntct gggtgcggca ggctcntggc ctcccctcca naagcgcctt tcccngnnga
      721 angctgcaca tcacgcagaa ggacanctac agggtctaca acacgatgcc ntgactcgag
      781 cncccccccn ccccnntgaa tcnggtgtt
//
