LOCUS       sequence-248517-        2899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target plasmid containing PSP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin
            EV, Zhang F
  TITLE     RNA-guided DNA insertion with CRISPR-associated transposases.
  JOURNAL   Science. 2019 Jul 5;365(6448):48-53. doi: 10.1126/science.aax9181. 
            Epub 2019 Jun 6.
  PUBMED    31171706
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aax9181"; journalName: "Science"; date: "2019-07-5-
            5"; volume: "365"; issue: "6448"; pages: "48-53"
COMMENT     Sequence Label: pTarget_CAST
FEATURES             Location/Qualifiers
     source          1..2899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(288..305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(422..967)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(502..521)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1583..1602)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(2033..2692)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2607..2626
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2693..2795)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt
       61 gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg
      121 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta tactggctta
      181 ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct
      241 gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac
      301 tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat
      361 ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt
      421 ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt
      481 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc
      541 gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt
      601 ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg
      661 cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      721 aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga
      781 gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg
      841 cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa
      901 accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg
      961 atctcaagaa gatcatctta ttaatatacg tagtatctac gatacgtagt atctacgata
     1021 cgtaggaggt tgagaagtca tttaataagg ccactgttaa acgtagatat atctacgcgt
     1081 agatatatct acgatacgta gcggccgacg cgctgggcta cgtcttaatg ggcagcgccc
     1141 acatacgcag cgatttccaa cagcttgagg cggaagcttt aatgcggtag tttatcacag
     1201 ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc
     1261 ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc
     1321 ctcttgcggg atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg
     1381 ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt
     1441 ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg
     1501 gcgaccacac ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc
     1561 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggcc
     1621 tgccaccata cccacgccga aacaagcgcc ctgcaccatt atgttccgga tctgcatcgc
     1681 aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc gctaaccgtt
     1741 tttatcaggc tctgggaggc agaataaatg atcatatcgt caattattac ctccacgggg
     1801 agagcctgag caaactggcc tcaggcattt gagaagcaca cggtcacact gcttccggta
     1861 gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa
     1921 ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta ttatcactta
     1981 ttcaggcgta gcaaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc
     2041 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     2101 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     2161 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     2221 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
     2281 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     2341 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     2401 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     2461 tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     2521 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     2581 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     2641 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     2701 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     2761 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg
     2821 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt
     2881 ccgtcacagg tatttattc
//
