LOCUS       sequence-24852-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Bissonnette SA, Glazier CM, Stewart MQ, Brown GE, Ellson CD, Yaffe 
            MB
  TITLE     Phosphatidylinositol 3-phosphate-dependent and -independent 
            functions of p40phox in activation of the neutrophil NADPH oxidase.
  JOURNAL   J Biol Chem. 2008 Jan 25. 283(4):2108-19.
  PUBMED    18029359
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jan 25. 283(4):2108-19."
COMMENT     Sequence Label: p40 R58Q/E259A
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             108..140
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 tgcgaattcc tctgaatatt ttgtttactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccatatg gctagcatga
      121 ctggtggaca gcaaatgggt cgcggatccg aattcatggc tgtggcccag cagctgcggg
      181 ccgagagtga ctttgaacag cttccggatg atgttgccat ctcggccaac attgctgaca
      241 tcgaggagaa gagaggcttc accagccact ttgttttcgt catcgaggtg aagacaaaag
      301 gaggatccaa gtacctcatc taccgccagt accgccagtt ccatgctttg cagagcaagc
      361 tggaggagcg cttcgggcca gacagcaaga gcagtgccct ggcctgtacc ctgcccacac
      421 tcccagccaa agtctacgtg ggtgtgaaac aggagatcgc cgagatgcgg atacctgccc
      481 tcaacgccta catgaagagc ctgctcagcc tgccggtctg ggtgctgatg gatgaggacg
      541 tccggatctt cttttaccag tcgccctatg actcagagca ggtgccccag gcactccgcc
      601 ggctccgccc gcgcacccgg aaagtcaaga gcgtgtcccc acagggcaac agctacngcn
      661 ctggnnncct ccnaaacaag nntttatttt ctncctgatg cncaactgga gctgatttca
      721 agctggagat gtgatcttct ctcatcgaca ccanatgctg gaggcctgtc gggaccnggc
      781 tntccnntct ttgnaanccn aa
//
