LOCUS       sequence-248531-        5966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Photoactivatable transcription system. Luciferase reporter under the
            control of the upstream activator sequence (UAS) of Gal4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: GAL4UAS-Luciferase reporter
FEATURES             Location/Qualifiers
     source          1..5966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      232..241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1921..2042
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1932..1951)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1986..2005
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2236..2593
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2236..2427
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2259..2279)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2444..2579
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2506..2525
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2624..3661
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             3640..3648
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    3685..3733
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(3889..3906)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4060..4648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4140..4159)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4848..5639,5640..5708))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5813..5861
                     /note="synthetic polyadenylation signal"
     misc_feature    5875..5966
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5915..5934
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtaccg agtttctaga cggagtactg tcctccgagc ggagtactgt
       61 cctccgactc gagcggagta ctgtcctccg atcggagtac tgtcctccgc gaattccgga
      121 gtactgtcct ccgaagacgc tagcgggggg ctataaaagg gggtgggggc gttcgtcctc
      181 actctagatc tgcgatctaa gtaagcttgg caatccggta ctgttggtaa agccaccatg
      241 gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc
      301 gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc
      361 tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt
      421 cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc
      481 agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct
      541 gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc
      601 cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag
      661 aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc
      721 caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc
      781 gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt
      841 accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat
      901 gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg
      961 ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg
     1021 gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag
     1081 attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc
     1141 gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag
     1201 gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc
     1261 ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca
     1321 gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca
     1381 ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac
     1441 gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc
     1501 gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg
     1561 atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac
     1621 cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc
     1681 gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat
     1741 gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag
     1801 gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag
     1861 gccaagaagt aaggccgcga ctctagagtc ggggcggccg gccgcttcga gcagacatga
     1921 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     1981 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     2041 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     2101 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tttgcgtatt
     2161 gggcgctctt ccgctgatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac
     2221 ttggttagct accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     2281 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     2341 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     2401 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     2461 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     2521 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     2581 aggcttttgc aaaaagctcg attcttctga cactagcgcc accatgaaga agcccgaact
     2641 caccgctacc agcgttgaaa aatttctcat cgagaagttc gacagtgtga gcgacctgat
     2701 gcagttgtcg gagggcgaag agagccgagc cttcagcttc gatgtcggcg gacgcggcta
     2761 tgtactgcgg gtgaatagct gcgctgatgg cttctacaaa gaccgctacg tgtaccgcca
     2821 cttcgccagc gctgcactac ccatccccga agtgttggac atcggcgagt tcagcgagag
     2881 cctgacatac tgcatcagta gacgcgccca aggcgttact ctccaagacc tccccgaaac
     2941 agagctgcct gctgtgttac agcctgtcgc cgaagctatg gatgctattg ccgccgccga
     3001 cctcagtcaa accagcggct tcggcccatt cgggccccaa ggcatcggcc agtacacaac
     3061 ctggcgggat ttcatttgcg ccattgctga tccccatgtc taccactggc agaccgtgat
     3121 ggacgacacc gtgtccgcca gcgtagctca agccctggac gaactgatgc tgtgggccga
     3181 agactgtccc gaggtgcgcc acctcgtcca tgccgacttc ggcagcaaca acgtcctgac
     3241 cgacaacggc cgcatcaccg ccgtaatcga ctggtccgaa gctatgttcg gggacagtca
     3301 gtacgaggtg gccaacatct tcttctggcg gccctggctg gcttgcatgg agcagcagac
     3361 tcgctacttc gagcgccggc atcccgagct ggccggcagc cctcgtctgc gagcctacat
     3421 gctgcgcatc ggcctggatc agctctacca gagcctcgtg gacggcaact tcgacgatgc
     3481 tgcctgggct caaggccgct gcgatgccat cgtccgcagc ggggccggca ccgtcggtcg
     3541 cacacaaatc gctcgccgga gcgcagccgt atggaccgac ggctgcgtcg aggtgctggc
     3601 cgacagcggc aaccgccggc ccagtacacg accgcgcgct aaggaggtag gtcgagttta
     3661 aactctagaa ccggtcatgg ccgcaataaa atatctttat tttcattaca tctgtgtgtt
     3721 ggttttttgt gtgttcgaac tagatgctgt cgaccgatgc ccttgagagc cttcaaccca
     3781 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     3841 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga
     3901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3961 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4021 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4081 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4141 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4201 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4261 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4321 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4381 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4441 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4501 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4561 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4621 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4681 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4741 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4801 gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg
     4861 atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt ggcctgactc
     4921 cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag cgcagcaatg
     4981 atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca gccagcaggg
     5041 agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc tatgagctgc
     5101 tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt tgtggccatt
     5161 gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc
     5221 cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt cagctcctta
     5281 gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca
     5341 gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt gaccggcgag
     5401 tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc ttgcccggcg
     5461 tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat catcgggaat
     5521 cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag ttcgatatag
     5581 cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt ttcggggtgt
     5641 gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg
     5701 atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta ctagtacgtc
     5761 tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc gcaataaaat
     5821 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata
     5881 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc
     5941 aagtgcaggt gccagaacat ttctct
//
