LOCUS       sequence-24854-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Bissonnette SA, Glazier CM, Stewart MQ, Brown GE, Ellson CD, Yaffe 
            MB
  TITLE     Phosphatidylinositol 3-phosphate-dependent and -independent 
            functions of p40phox in activation of the neutrophil NADPH oxidase.
  JOURNAL   J Biol Chem. 2008 Jan 25. 283(4):2108-19.
  PUBMED    18029359
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jan 25. 283(4):2108-19."
COMMENT     Sequence Label: p40 D293K/E259A
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..105
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             109..141
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 tgngaattcc ntctnaatat tttgtttact ttaagaagga gatataccat gggcagcagc
       61 catcatcatc atcatcacag cagcggcctg gtgccgcgcg gcagccatat ggctagcatg
      121 actggtggac agcaaatggg tcgcggatcc gaattcatgg ctgtggccca gcagctgcgg
      181 gccgagagtg actttgaaca gcttccggat gatgttgcca tctcggccaa cattgctgac
      241 atcgaggaga agagaggctt caccagccac tttgttttcg tcatcgaggt gaagacaaaa
      301 ggaggatcca agtacctcat ctaccgccgc taccgccagt tccatgcttt gcagagcaag
      361 ctggaggagc gcttcgggcc agacagcaag agcagtgccc tggcctgtac cctgcccaca
      421 ctcccagcca aagtctacgt gggtgtgaaa caggagatcg ccgagatgcg gatacctgcc
      481 ctcaacgcct acatgaagag cctgctcagc ctgccggtct gggtgctgat ggatgaggac
      541 gtccggatct tcttttacca gtcgccctat gactcagagc aggtgcccca ggcactccgc
      601 cggctccgcc cgcgcacccg gaaagtcaag agcgtgtccc cacagggcaa cagcgttgac
      661 cgcatggcag ctccgagagc agaggctcta tttgacttca ctggaaacag caaactggag
      721 ctganttcaa gctggaangt gatcttcctc ctcagtcgan caacntgaat ggctggaggc
      781 actgtcnggg agcacggcnn ttcctcntct tctgagnctc aana
//
