LOCUS       sequence-248555-        2448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConLS and chicken hypersensitivity site 4 insulator as a 
            Type 1 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_008
FEATURES             Location/Qualifiers
     source          1..2448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     416..435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1429..1463)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1534..2193)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2108..2127
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2375..2448,1..515)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
       61 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
      121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      301 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      601 ctgtggataa ccgtagtcgg tctcaccctg aattcgcatc tagatggtag agccacaaac
      661 agccggtaca agcaacgatc tccaggacca tctgaatcat gcgcggatga cacgaactca
      721 cgacggcgat cacagacatt aacccacagt acagacactg cgacaacgtg gcaattcgtc
      781 gcaataccgt ctcactgaac tggccgataa ttgcagacga tcgaattcga gttggcgcgc
      841 ctgtcattct aaatctctct ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct
      901 gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg
      961 tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag
     1021 cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg
     1081 ggggctttgg gagggggctg tccccgtgag ctcccaggcg cgcctgtcat tctaaatctc
     1141 tctttcagcc taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag
     1201 cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg
     1261 cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct
     1321 cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg
     1381 ctgtccccgt gagctcaata aaagagccca caacgtgaga ccagaccaat aaaaaacgcc
     1441 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     1501 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
     1561 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     1621 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     1681 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     1741 cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc
     1801 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     1861 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1921 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     1981 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     2041 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     2101 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     2161 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     2221 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     2281 tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2341 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2401 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtt
//
