LOCUS       sequence-248561-        2448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConL2 and chicken hypersensitivity site 4 insulator as a 
            Type 1 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_010
FEATURES             Location/Qualifiers
     source          1..2448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     302..321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1315..1349)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1420..2079)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1994..2013
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2261..2448,1..401)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
       61 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      421 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      481 ctgattctgt ggataaccgt agtcggtctc accctgaatt cgcatctaga ataaatcgca
      541 gccaagtgag tgaatagatg acgcaccacg gtcagacacg gccacatcgt atctcacagg
      601 agcaagcgcg ataggagcac tcacacatag tacggtgatc cgctgactcc tttgcccaaa
      661 taagacgtga gcccgtctca gatgcacaca ctggcttaag atgacatcga attcgagttg
      721 gcgcgcctgt cattctaaat ctctctttca gcctaaagct ttttccccgt atccccccag
      781 gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc cgtgccacct
      841 tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg gagcgccgga
      901 ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga cgtaattaca
      961 tccctggggg ctttgggagg gggctgtccc cgtgagctcc caggcgcgcc tgtcattcta
     1021 aatctctctt tcagcctaaa gctttttccc cgtatccccc caggtgtctg caggctcaaa
     1081 gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg
     1141 tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg
     1201 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg
     1261 ggggggctgt ccccgtgagc tcaataaaag agcccacaac gtgagaccag accaataaaa
     1321 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     1381 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
     1441 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
     1501 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     1561 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     1621 gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa
     1681 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     1741 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     1801 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     1861 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     1921 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     1981 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     2041 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     2101 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     2161 ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2221 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2281 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2341 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2401 atactgttct tctagtgtag ccgtagttag gccaccactt caagaact
//
