LOCUS       sequence-248565-        2448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConL3 and chicken hypersensitivity site 4 insulator as a 
            Type 1 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_011
FEATURES             Location/Qualifiers
     source          1..2448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     423..442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1436..1470)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1541..2200)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2115..2134
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2382..2448,1..522)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
       61 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      121 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      181 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      241 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      301 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      361 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      421 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      481 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      541 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      601 cctgattctg tggataaccg tagtcggtct caccctgaat tcgcatctag agcagaccta
      661 gaccacatga ggctgatgta ggacagccac cagtggcagc taatcaacac cgcaagatgc
      721 cgatgcacgc tcatatcatc gtcgtcagcc tggtagccat tcgacatacg gatcagggaa
      781 ctcgaaccca gtaccgtctc agttctgtat gggcacagac aacctaatcg aattcgagtt
      841 ggcgcgcctg tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca
      901 ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc
      961 ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg
     1021 accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac
     1081 atccctgggg gctttgggag ggggctgtcc ccgtgagctc ccaggcgcgc ctgtcattct
     1141 aaatctctct ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa
     1201 agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct
     1261 gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg
     1321 ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg
     1381 gggggggctg tccccgtgag ctcaataaaa gagcccacaa cgtgagacca gaccaataaa
     1441 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1501 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     1561 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1621 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1681 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1741 tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga
     1801 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     1861 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     1921 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     1981 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     2041 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     2101 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     2161 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     2221 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     2281 tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc
     2341 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2401 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtg
//
