LOCUS       sequence-248566-        2448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConL4 and chicken hypersensitivity site 4 insulator as a 
            Type 1 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_012
FEATURES             Location/Qualifiers
     source          1..2448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     297..316
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1310..1344)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1415..2074)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1989..2008
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2256..2448,1..396)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
       61 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      121 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      181 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      241 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      301 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      361 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      421 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      481 tctgtggata accgtagtcg gtctcaccct gaattcgcat ctagagcctg cactagacga
      541 actaggcaag atgcgtccaa tccgtctaaa catggtgaca acgctggaca gatgacgtaa
      601 caccgagcca catcctgaaa tcgaggcagg ctaaccgaaa ccgtgacaat gcaaagagac
      661 agcctgaccg tctcaggtag actacccatg agtcacaatg atcgaattcg agttggcgcg
      721 cctgtcattc taaatctctc tttcagccta aagctttttc cccgtatccc cccaggtgtc
      781 tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc
      841 gtgcccgggc tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga
      901 gcggagcccc gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct
      961 gggggctttg ggagggggct gtccccgtga gctcccaggc gcgcctgtca ttctaaatct
     1021 ctctttcagc ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc tcaaagagca
     1081 gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc
     1141 gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc cccgggcggc
     1201 tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct ttgggggggg
     1261 gctgtccccg tgagctcaat aaaagagccc acaacgtgag accagaccaa taaaaaacgc
     1321 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1381 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
     1441 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1501 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1561 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     1621 tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta gggaaatagg
     1681 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     1741 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     1801 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     1861 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     1921 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     1981 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     2041 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     2101 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     2161 ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc gttccactga
     2221 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2281 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2341 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2401 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgta
//
