LOCUS       sequence-248569-        2482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConLS, chicken hypersensitivity site 4 insulator and locus 
            of X-over P1 (loxP) as a Type 1 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_015
FEATURES             Location/Qualifiers
     source          1..2482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     416..435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1412..1445
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(1463..1497)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1568..2227)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2142..2161
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2409..2482,1..515)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
       61 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
      121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      301 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      601 ctgtggataa ccgtagtcgg tctcaccctg aattcgcatc tagatggtag agccacaaac
      661 agccggtaca agcaacgatc tccaggacca tctgaatcat gcgcggatga cacgaactca
      721 cgacggcgat cacagacatt aacccacagt acagacactg cgacaacgtg gcaattcgtc
      781 gcaataccgt ctcactgaac tggccgataa ttgcagacga tcgaattcga gttggcgcgc
      841 ctgtcattct aaatctctct ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct
      901 gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg
      961 tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag
     1021 cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg
     1081 ggggctttgg gagggggctg tccccgtgag ctcccaggcg cgcctgtcat tctaaatctc
     1141 tctttcagcc taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag
     1201 cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg
     1261 cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct
     1321 cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg
     1381 ctgtccccgt gagctcaata aaagagccca cataacttcg tataatgtat gctatacgaa
     1441 gttataacgt gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     1501 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     1561 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     1621 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1681 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1741 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa
     1801 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1861 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1921 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     1981 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     2041 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     2101 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     2161 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     2221 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     2281 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aatcatgacc
     2341 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2401 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2461 ccgctaccag cggtggtttg tt
//
