LOCUS       sequence-248573-        1856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConL8 as a Type 1 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_018
FEATURES             Location/Qualifiers
     source          1..1856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(221..255)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(326..985)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     900..919
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcac cctgaattcg catctagagg aatgtacgga acctcgatgc cattgcatct
       61 gaacgcctgg cgatcagacg acactagagt cacgacgata ccaggtctgt catatgagcg
      121 gaacccatgc cagattgacc caactccatg aaactacgat gcagagactg gcgtctcatt
      181 tatatatgga gtcccgccag cataacgtga gaccagacca ataaaaaacg cccggcggca
      241 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
      301 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
      361 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
      421 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
      481 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
      541 gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
      601 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
      661 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
      721 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
      781 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
      841 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
      901 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
      961 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     1021 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1081 gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtag
//
