LOCUS       sequence-248579-        2448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConL6 and chicken hypersensitivity site 4 insulator as a 
            Type 1 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_019
FEATURES             Location/Qualifiers
     source          1..2448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     496..515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1509..1543)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1614..2273)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2188..2207
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
       61 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
      121 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
      181 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
      241 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
      301 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      361 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      421 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      481 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      541 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      601 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      661 tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg tctcaccctg aattcgcatc
      721 tagaacgggc tgaatcacaa gcatccaaag ctcgtcgaat agttggcgag agtaaggaat
      781 gccacccgcg atcagaacag atgccgaaga tggcactcac gtcactggca tgtcctaaag
      841 ctgccgagat ttgcgaacag cgatccccgt ctcaccctag ggacactgac acaattccaa
      901 tcgaattcga gttggcgcgc ctgtcattct aaatctctct ttcagcctaa agctttttcc
      961 ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg
     1021 atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg
     1081 gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga
     1141 gggacgtaat tacatccctg ggggctttgg gagggggctg tccccgtgag ctcccaggcg
     1201 cgcctgtcat tctaaatctc tctttcagcc taaagctttt tccccgtatc cccccaggtg
     1261 tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc
     1321 ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg
     1381 gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc
     1441 ctgggggctt tggggggggg ctgtccccgt gagctcaata aaagagccca caacgtgaga
     1501 ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     1561 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     1621 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     1681 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     1741 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     1801 aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata
     1861 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     1921 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     1981 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     2041 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     2101 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     2161 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     2221 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     2281 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     2341 ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg
     2401 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaagg
//
