LOCUS       sequence-248581-        2448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConL7 and chicken hypersensitivity site 4 insulator as a 
            Type 1 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_020
FEATURES             Location/Qualifiers
     source          1..2448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     449..468
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1462..1496)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1567..2226)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2141..2160
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2408..2448,1..548)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
       61 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      121 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      181 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      241 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      301 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      361 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      421 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      481 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      541 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      601 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcacc
      661 ctgaattcgc atctagacca gacagcacgt aattgaccgc cagaacgcgc aatttgacga
      721 ccagcggata tgagattctg tgcactcacg agaacgttac cgacgatttc ccagtatttg
      781 gcgctgccat aatccaaata tcaagcgaat cccatgtgac cgtctcagcg ggcgacgatc
      841 tcagaacagt taatcgaatt cgagttggcg cgcctgtcat tctaaatctc tctttcagcc
      901 taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
      961 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
     1021 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
     1081 ccctagcggg ggagggacgt aattacatcc ctgggggctt tgggaggggg ctgtccccgt
     1141 gagctcccag gcgcgcctgt cattctaaat ctctctttca gcctaaagct ttttccccgt
     1201 atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc
     1261 cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg
     1321 gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga
     1381 cgtaattaca tccctggggg ctttgggggg gggctgtccc cgtgagctca ataaaagagc
     1441 ccacaacgtg agaccagacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     1501 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     1561 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1621 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1681 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1741 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg aaacgaaaaa
     1801 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1861 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1921 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1981 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     2041 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     2101 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     2161 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     2221 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     2281 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca
     2341 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2401 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaa
//
