LOCUS       sequence-248582-        2020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConLS and the human A4 insulator as a Type 1 part to be used
            in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_022
FEATURES             Location/Qualifiers
     source          1..2020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     416..435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1001..1035)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1106..1765)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1680..1699
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1947..2020,1..515)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
       61 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
      121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      301 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      601 ctgtggataa ccgtagtcgg tctcaccctg aattcgcatc tagatggtag agccacaaac
      661 agccggtaca agcaacgatc tccaggacca tctgaatcat gcgcggatga cacgaactca
      721 cgacggcgat cacagacatt aacccacagt acagacactg cgacaacgtg gcaattcgtc
      781 gcaataccgt ctcactgaac tggccgataa ttgcagacga ggagcatcgc ccttccccgg
      841 ccctcaggta agaggaccaa ataccgtagc cgtttccaat ttcagtcctt tagcgccacc
      901 tggtgctaac tactctatca cgcttttatc caataactac ctttgtaaat ttcctttcaa
      961 aagttctggc cgggcgcggt ggcaacgtga gaccagacca ataaaaaacg cccggcggca
     1021 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
     1081 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
     1141 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     1201 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     1261 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     1321 gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     1381 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     1441 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     1501 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
     1561 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1621 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     1681 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     1741 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     1801 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1861 gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1921 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1981 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
//
