LOCUS       sequence-248586-        2061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConL4 and human E1 insulator as a Type 1 part to be used in 
            the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_026
FEATURES             Location/Qualifiers
     source          1..2061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     297..316
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(923..957)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1028..1687)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1602..1621
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1869..2061,1..396)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
       61 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      121 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      181 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      241 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      301 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      361 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      421 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      481 tctgtggata accgtagtcg gtctcaccct gaattcgcat ctagagcctg cactagacga
      541 actaggcaag atgcgtccaa tccgtctaaa catggtgaca acgctggaca gatgacgtaa
      601 caccgagcca catcctgaaa tcgaggcagg ctaaccgaaa ccgtgacaat gcaaagagac
      661 agcctgaccg tctcaggtag actacccatg agtcacatga aggagtgggc ctggaataaa
      721 accagaaatc cccattctca gtctgcccca agccctccaa ttccgagggc gctgtatgta
      781 taagctcggg caggcaaaag tcgactgtga gaacacgcca gcagagggcg ctgtggcccc
      841 atcagtccct gcccactgaa ctctccagaa ggaaaagcgg caaggatgca aacaagaaaa
      901 tcagaaacgt gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
      961 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     1021 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     1081 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1141 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1201 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa
     1261 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1321 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1381 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     1441 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     1501 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1561 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1621 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     1681 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     1741 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aatcatgacc
     1801 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1861 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1921 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1981 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2041 caccacttca agaactctgt a
//
