LOCUS       sequence-248587-        2093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConL5 and human E4 insulator as a Type 1 part to be used in 
            the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_027
FEATURES             Location/Qualifiers
     source          1..2093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     156..175
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      423..1011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     912..931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1570..1604)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1675..2093,1..241))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
       61 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
      121 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
      181 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
      241 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
      301 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc
      361 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
      421 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
      481 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
      541 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
      601 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
      661 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
      721 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      781 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      841 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      901 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      961 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1021 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     1081 gcgttatccc ctgattctgt ggataaccgt agtcggtctc accctgaatt cgcatctaga
     1141 acgcacaagg tcagggcact catgcgacaa tcaactcgat gcatgatccg caccattgtc
     1201 gaggggccag cgtcaatagt gccgatgacc acagacccgg ttaagacata gccgaatgga
     1261 gccgcgccga ccacagaatg atacgtctca aagtgaataa agctccacac agtcgatgtt
     1321 tctatatgtt ttaagtctta ggcatatcgg acatcactag tgcatcagcg ccctctgctg
     1381 gttcagtaag aatggtttcc ccatatactg ggcaaaactg gatttttgtg gtgatgaaag
     1441 ggaaaaaaac aaattcagta caattggaaa gctggtgttg ttttaaaact cttgaaaaac
     1501 actgaatgaa aggaatcaca ctaaaactat atgttgcaat gttgttggtt aaaacgtgag
     1561 accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     1621 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     1681 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     1741 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     1801 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     1861 taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat
     1921 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     1981 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     2041 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gct
//
