LOCUS       sequence-248591-        2000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConL7 and human B2 insulator as a Type 1 part to be used in 
            the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_029
FEATURES             Location/Qualifiers
     source          1..2000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     449..468
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1014..1048)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1119..1778)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1693..1712
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1960..2000,1..548)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
       61 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      121 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      181 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      241 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      301 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      361 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      421 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      481 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      541 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      601 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcacc
      661 ctgaattcgc atctagacca gacagcacgt aattgaccgc cagaacgcgc aatttgacga
      721 ccagcggata tgagattctg tgcactcacg agaacgttac cgacgatttc ccagtatttg
      781 gcgctgccat aatccaaata tcaagcgaat cccatgtgac cgtctcagcg ggcgacgatc
      841 tcagaacagt tacaacacca ggcttctggc tccagtcctt ggagtccgtc tgcgtcccag
      901 cagctccccc tggtggccag aagcaaacgc ttcatgtctt gcatctagag ccaaagcatc
      961 tcctacccct ggattcccat tccggaatag ttttcaaacg tgagaccaga ccaataaaaa
     1021 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     1081 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
     1141 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     1201 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     1261 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     1321 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa
     1381 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     1441 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     1501 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     1561 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     1621 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     1681 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     1741 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     1801 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     1861 gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca
     1921 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1981 cgtaatctgc tgcttgcaaa
//
