LOCUS       sequence-248594-        1905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConL3 and picornavius peptide 2A (P2A) element as a Type 1 
            part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_032
FEATURES             Location/Qualifiers
     source          1..1905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     423..442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             817..873
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     terminator      complement(893..927)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(998..1657)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1572..1591
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1839..1905,1..522)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
       61 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      121 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      181 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      241 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      301 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      361 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      421 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      481 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      541 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      601 cctgattctg tggataaccg tagtcggtct caccctgaat tcgcatctag agcagaccta
      661 gaccacatga ggctgatgta ggacagccac cagtggcagc taatcaacac cgcaagatgc
      721 cgatgcacgc tcatatcatc gtcgtcagcc tggtagccat tcgacatacg gatcagggaa
      781 ctcgaaccca gtaccgtctc agttcgggga tcaggtgcaa caaatttctc actactcaaa
      841 caggcaggag atgtagaaga aaatccagga ccgggaacgt gagaccagac caataaaaaa
      901 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
      961 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     1021 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     1081 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     1141 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     1201 aactcaccca gggattggct gaaacgaaaa acatattctc aataaaccct ttagggaaat
     1261 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     1321 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     1381 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     1441 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     1501 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     1561 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     1621 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     1681 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     1741 aacctcttac gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1801 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1861 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtg
//
