LOCUS       sequence-248596-        1905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConL5 and picornavius peptide 2A (P2A) element as a Type 1 
            part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_034
FEATURES             Location/Qualifiers
     source          1..1905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     156..175
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      423..1011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     912..931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1306..1362
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     terminator      complement(1382..1416)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1487..1905,1..241))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
       61 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
      121 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
      181 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
      241 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
      301 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc
      361 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
      421 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
      481 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
      541 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
      601 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
      661 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
      721 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      781 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      841 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      901 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      961 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1021 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     1081 gcgttatccc ctgattctgt ggataaccgt agtcggtctc accctgaatt cgcatctaga
     1141 acgcacaagg tcagggcact catgcgacaa tcaactcgat gcatgatccg caccattgtc
     1201 gaggggccag cgtcaatagt gccgatgacc acagacccgg ttaagacata gccgaatgga
     1261 gccgcgccga ccacagaatg atacgtctca aagtggggta gtggagccac gaatttcagt
     1321 ttattaaaac aggctggcga tgtcgaagag aaccctgggc caggaacgtg agaccagacc
     1381 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     1441 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     1501 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     1561 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     1621 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     1681 aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt
     1741 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     1801 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     1861 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagct
//
