LOCUS       sequence-248604-        2096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pSV40 as a Type 2 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK2_006
FEATURES             Location/Qualifiers
     source          1..2096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      89..677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     578..597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(857..877)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        862..1191
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1042..1177
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1104..1123
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     terminator      complement(1239..1273)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1344..2003)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1918..1937
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
       61 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      301 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      421 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      661 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      721 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtagtc
      781 ggtctcaaac gattacgcca agctggccgg cccgtacggc cacgcggatc tcgacattga
      841 ttattgacta gtctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
      901 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
      961 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     1021 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     1081 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     1141 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     1201 ggagcttgta tatccagcca ctatgtgaga ccagaccaat aaaaaacgcc cggcggcaac
     1261 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     1321 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     1381 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     1441 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     1501 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     1561 ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     1621 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     1681 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     1741 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     1801 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     1861 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     1921 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     1981 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     2041 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttac
//
