LOCUS       sequence-248606-        2847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human pEF1alpha as a Type 2 part to be used in the MTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK2_007
FEATURES             Location/Qualifiers
     source          1..2847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      89..677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     578..597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        804..1015
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          1016..1954
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1911..1931
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     terminator      complement(1990..2024)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2095..2754)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2669..2688
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
       61 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      301 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      421 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      661 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      721 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtagtc
      781 ggtctcaaac ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
      841 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
      901 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
      961 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     1021 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     1081 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     1141 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     1201 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     1261 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     1321 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     1381 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     1441 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     1501 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     1561 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     1621 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     1681 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     1741 cgattagttc tcgcgctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     1801 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     1861 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     1921 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgagctagcc actatgtgag
     1981 accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     2041 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     2101 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     2161 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     2221 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     2281 taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat
     2341 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     2401 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     2461 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     2521 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     2581 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     2641 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     2701 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     2761 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     2821 attatggtga aagttggaac ctcttac
//
