LOCUS       sequence-248612-        1969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pSynTF (miniCMV core) as a Type 2 part to be used in the MTK
            sytem.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK2_015
FEATURES             Location/Qualifiers
     source          1..1969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      142..730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     631..650
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1096..1120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     terminator      complement(1165..1199)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1270..1929)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1844..1863
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg
       61 atcaatcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
      121 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
      181 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
      241 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
      301 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
      361 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      421 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      481 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      541 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      601 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      661 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      721 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      781 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta gtcggtctca
      841 aacgcgggtt tcgtaacaat cgcatgagga ttcgcaacgc cttcgtcgaa gtcgaagtcg
      901 acctccagtc tcagtaaagg tcgtcgaagt cgaagtcgac caatcggact gccttcgtac
      961 gtcgaagtcg aagtcgaccg tatcagtcgc ctcggaacgt cgaagtcgaa gtcgaccatt
     1021 cgtaagaggc tcactctccc ttacacggag tggataacta gttaggcgtg tacggtggga
     1081 ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcctggaacg cgtaccggtg
     1141 tcgccactat gtgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     1201 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     1261 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     1321 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     1381 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     1441 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa
     1501 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     1561 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     1621 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     1681 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     1741 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     1801 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     1861 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     1921 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaa
//
