LOCUS       sequence-248614-        1728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes truncated pCMV as a Type 2 part to be used in the MTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK2_016
FEATURES             Location/Qualifiers
     source          1..1728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      89..677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     578..597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(871..905)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(976..1635)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1550..1569
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
       61 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      301 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      421 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      661 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      721 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtagtc
      781 ggtctcaaac gggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
      841 taccggtcgc cactatgtga gaccagacca ataaaaaacg cccggcggca accgagcgtt
      901 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
      961 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     1021 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     1081 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     1141 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     1201 aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     1261 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     1321 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     1381 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     1441 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     1501 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     1561 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     1621 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     1681 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttac
//
