LOCUS       sequence-248616-        1758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes truncated pCMV as a Type 2 part to be used in the MTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK2_018
FEATURES             Location/Qualifiers
     source          1..1758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      57..91
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     promoter        complement(152..196)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     rep_origin      complement(315..903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(395..414)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1085..1744
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1151..1170)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
       61 gaacgctcgg ttgccgccgg gcgtttttta ttggtctggt ctcacatagt ggcgaccggt
      121 agcgctagcg gatctgacgg ttcactaaac cagctctgct tatatagacc tcccaccgta
      181 cacgcctacc gcccatttgc cgtttgagac cgactacggt tatccacaga atcaggggat
      241 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      301 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      361 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      421 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      481 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      541 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      601 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      661 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      721 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      781 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
      841 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
      901 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
      961 taagggattt tggtcatgat tgatcgggca cgtaagaggt tccaactttc accataatga
     1021 aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc
     1081 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     1141 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     1201 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     1261 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga
     1321 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     1381 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     1441 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     1501 tgagaatatg tttttcgttt cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     1561 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     1621 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     1681 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     1741 gtaatttgat atcgagct
//
