LOCUS       sequence-248617-        9926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  7886 bases long scaffold with Zn-dependent self-cleaving DNAzyme 
            cassettes, from compatibility group 8, made by method IV, yields 
            linear 7569 b long scaffolds after Zn incubation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9926)
  AUTHORS   Engelhardt FAS, Praetorius F, Wachauf CH, Bruggenthies G, Kohler F, 
            Kick B, Kadletz KL, Pham PN, Behler KL, Gerling T, Dietz H
  TITLE     Custom-Size, Functional, and Durable DNA Origami with 
            Design-Specific Scaffolds.
  JOURNAL   ACS Nano. 2019 Apr 22. doi: 10.1021/acsnano.9b01025.
  PUBMED    30990672
REFERENCE   2  (bases 1 to 9926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Nano. 
            2019 Apr 22. doi: 10.1021/acsnano.9b01025."
COMMENT     Sequence Label: pre_IV_CS8_7569_v1
FEATURES             Location/Qualifiers
     source          1..9926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7079..7090
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      complement(8101..8689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8181..8200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8860..9651,9652..9720))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9483..9502
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9721..9825)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9893..9911
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
       61 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggcaagct tgtcacttgg
      121 acactagttg agctgtcaca gaatgtgacg ttgaaggtgt ccaagctgtc aacgcaccaa
      181 gcatacaagg gtgtaagctg agaacctcat aacgctagaa catcataaga gcgcaacacg
      241 ataatccagg aacctgacaa ggtagtaacc ccctaatccc acaagagggt aacacctcaa
      301 cagggcaact cgtgaatgtc tgaagtagat aacctgataa ggggctaagc cactaagccc
      361 ctaagcgccc aatggcgtaa tgtgacaata cggtaagtcc tcaagagtcc aacgtagcaa
      421 ccgtcgaaca cgctaacatc ggaacactac aatacgataa cccaggaacg agggaactag
      481 cgaacagcgt aactgtggaa tcgggcaatg cggtaatcga tcaacatggt aatctacgaa
      541 ctccacaatg cagcaacgac ctaagcagat aatatgagaa cgtggcaaca tctgaatggt
      601 agaacgctgc aaggaggtaa cgagctaatc cggtaagggg ggaacggagg aaccgaccaa
      661 tacatgaagg atgtaataga ggaagctgac aaggcaccaa gtgtcgaaga tcgcaatgag
      721 ggaatatatc aatccagtaa gggcgcaagc ggataacatg cgaacgcctg aatggggcaa
      781 ccgcgcaatc ggtcaatggt cgaacgctac aagtggacaa caggcgaagt gctcaactga
      841 gtaaggatcg aatccatgaa gccatgaact atctaatccc gcaactccat aacgtcgtaa
      901 gatgcgaagc gtgtaaggtg acaatatccc aagatagcaa gtatataagg gcggaagcca
      961 ggaagctggc aacgcgcgaa tcgtgtaagt gagtaagtgc ggaagacacg aacgacacaa
     1021 tgtgggaacc tcagaacgct gtaacgatcc aatctgataa ccacgtaagt acctaaggcg
     1081 tgaacacccg aatgtcagaa tgcctcaatc agagaatcac ctaatctagc aatcccccaa
     1141 cgccgtaacc acccaagccg cgaagatacc aagagtataa tcgaggaatg cgataacccc
     1201 cgaagggtgg aatcacagaa ctcgctaatg ctccaagtgc acaactctct aactgtgtaa
     1261 gctcgtaatc cgcgaacatg ccaactgtcc aaccctccaa tatcagaaga ggtcaagtct
     1321 ctaaccgact aacggtacaa tcatagaatg ggataacggg ctaagggacc aagtacgtaa
     1381 tcgcacaatg acgtaagggg acaatgagag aagccgtcaa gcctgcaatc ggcgaaccta
     1441 ccaagagatg aactggggaa cgatcgaatg taccaacact ataaggcctg aatgtcccaa
     1501 gcgcataaca cagcaagtgc gtaatacgcg aactgtacaa ggccggaact ctggaaccag
     1561 gtaaccatcg aatgctgcaa cagtagaagc tgccaagctg ggaacacggg aaggagataa
     1621 tatgtgaatg agataacagt ccaagtgatg aagtagccaa ggccgcaact ctacaagtca
     1681 gcaatggcgg aacaccctaa ctacccaact cactaacccg agaagggggc aagcccataa
     1741 cggagtaatc ggagaatctc tgaaccacag aatagcctaa tatctcaaca cgcgaatgcc
     1801 agaagagcac aagcatgcaa tcgactaagg acgtaacact gtaatgaggc aatgccgtaa
     1861 gaccccaagg ccgtaatacg agaacagggt aactgtctaa cgagacaagc gcagaactca
     1921 ccaagtgtct aagcatggaa tatcataaga cctgaaggcg cgaaccagcg aaccatccaa
     1981 catcgcaacg tgataataca gtaactctcc aactaccgaa tctggcaatc gacgaatcgc
     2041 gtaatgacgc aaccgggtaa ggtcagaaga tgacaagctc ccaatgatac aagcagtgaa
     2101 cgagataagg gtcgaatcgg ctaacaggtg aaccatggaa ctagggaatg gtacaagtcg
     2161 cgaagtactg aacgcaggaa gtgagcaacc cgacaacatc acaatcgtct aaggctcgaa
     2221 tggatcaatc gctgaactgc tcaagcgccg aatgcatcaa cgacgtaacg ggataatgtg
     2281 cgaagcggac aatagtctaa tccgagaatc agcgaacgcc acaatagctg aagatggtaa
     2341 gggtacaatc tcgtaagtag ctaacgtgtc aacgcgataa tgaggtaaga cgagaacagg
     2401 acaataccat aatagtgcaa taccacaagg cgacaatgta ctaagacggt aagtgtataa
     2461 tcagctaagc gcggaaccta ggaatggcct aatgtggtaa gacatgaagt atgcaagcgc
     2521 tcaaggtacc aagacgataa tcgaccaagg tcgtaacagc ccaatccctc aaccgctcaa
     2581 ccagagaaca tgctaactgg cgaagcccgc aactcatcaa ggtgccaatg atataatcgc
     2641 ctaacatggc aaccggcgaa tatagtaaca gagtaactgc ccaaccgggg aagtcaccaa
     2701 gcagggaagg cacgaagtat ggaacgggcg aatcccgtaa taggtcaatc tgccaagagt
     2761 agaagcactc aagacccgaa cgtcataact cgcgaatatc tgaacggcac aataggataa
     2821 ggatccaagg ggagaatacc tcaacgcagc aaccgcgtaa gtctcgaaga catcaagtag
     2881 gcaagcagct aagcgcacaa cctagcaagg atacaatgtc tcaactagct aacgaggtaa
     2941 cgccataatc aggtaacccg ataactggtc aaccccccaa gacgcgaagc atctaatgtc
     3001 ggaacgcggg aatcggccaa cctatcaatg ccggaatgca cgaacgcgtg aagctacgaa
     3061 taggggaatc tcgcaagtgg tcaatactcc aacggtctaa tactcgaaca tagcaatgcc
     3121 gcaactagtc aatataccaa ctgatcaaca tctcaatggg cgaagatgtg aaccccacaa
     3181 tccgccaaga tagtaagtgg cgaactcagg aatggagtaa ggtgcgaata gagtaactcc
     3241 gtaacgagcg aagacgggaa ccccataatc gtcgaaggca tcaatcggtg aaggggccaa
     3301 ggcggtaagc acacaacagg ggaaggacct aagaggtgaa gagccgaacg gtgcaagtga
     3361 cgaacatgtc aagcacgcaa cgctctaagc ggggaatgcg gcaacccagc aaccctacaa
     3421 gactccaaca cgagaagctg tgaaggagag aagcgatgaa ctgaccaagt ctccaaccac
     3481 ctaacgactg aatatactaa tgagtcaagc acagaagact gtaactctgt aagctagcaa
     3541 ctcggtaatc ggggaaccca gtaatagatg aatgagccaa ccgcagaata caggaacctc
     3601 ccaagcctcc aacgccgcaa ggtggtaacg gggcaatagt ggaagaccac aacgccccaa
     3661 cgtatcaacc gtctaatgta cgaagaccat aatcgtgcaa cagtataatc atcgaacact
     3721 ccaactatcg aagctgataa gaccggaagt atgtaaggca gcaagtgcgc aatgtgctaa
     3781 ccatctaagc gtccaaggct ggaaccggtg aaggccccaa cgcgagaacc ccgcaacctg
     3841 ctaacaccgg aatgatgtaa cgccctaagg tgtgaagatc gtaagacggc aatgtcgtaa
     3901 caggtcaagt cgggaacgcg gtaagcggct aactgtataa cctccgaatg atgcaactca
     3961 cgaacggact aaccccagaa tggactaacg tacgaaggat ctaagagggc aaggccagaa
     4021 gggcataagc tgctaagaga gtaagacgcc aatctgtgaa ctgacgaatc atggaagctg
     4081 gtaatccggc aaggagcgaa catactaatc gcgcaagagt cgaagccgac aagtgatcaa
     4141 cgacggaagg gactaactcc cgaaggtcgc aatgacagaa gcggcgaata ggacaacctg
     4201 gtaacatgag aagaccagaa tagtacaacc tagtaacact ctaacctatg aatagcataa
     4261 tgcgtcaata ccccaagatc ccaatggagc aacaccagaa gatcagaagc aggcaatgat
     4321 ctaatgactc aacgctcgaa cctgagaagt ggccaatata gcaacgtatg aatcatataa
     4381 tagtagaata gatcaacgat acaagactgc aatcgtagaa tcgcataaca ccataagcac
     4441 ggaagtgctg aacacaggaa tacggcaacc cgcgaatcgg ataacacacg aacagctcaa
     4501 gctcggaagt cgccaacctc tgaagcagac aaggcactaa tcagggaacg cctcaaggga
     4561 ggaacggggt aagacctcaa cctgtgaacc cctgaacccg ccaacgatct aaccgacgaa
     4621 gatgctaagt cacgaatatg ctaaggtctg aatccgacaa tgagctaaca gaggaacagg
     4681 ctaacatgat aacgcgctaa tcctccaata cgtgaatgcc ataacgtctg aatgccccaa
     4741 gctccgaatc tgacaaggac gcaatctctc aagacaccaa gtcccgaata cgtcaagtcc
     4801 acaaggccct aagtggtgaa tgacggaatg gtgtaataca ccaaccatgc aatgcgcgaa
     4861 tgctacaagg gcacaagtac ccaacggatg aactaggcaa cagcagaacc tgcgaagatg
     4921 ggaaccacac aagtgaggaa ctcgagaatg ctggaacgac ataatgtatg aacccactaa
     4981 ctcagcaaca ggccaagccc tgaagatggc aacgtgctaa gtgccgaatc cctgaagagc
     5041 ataatgagcg aaggcaggaa tcacgtaact agataactcg ataagggatg aactgatgaa
     5101 gcgactaatc agacaacgcg tcaacgtcgg aatcgtccaa ccctgtaagt gcctaacacc
     5161 gcaacatcag aactacctaa gatactaacg gtataagacg acaatggtat aataccagaa
     5221 ctgccgaagc tgcgaaggag tgaatagggc aaccactcaa cagagcaagg acacaagcat
     5281 ccaagccggc aagcagccaa tggacgaatc gcagaacggc agaagctcat aagtcggtaa
     5341 tgcccgaact ccctaacggc ataacacgtg aagatcacaa tgtggcaagg cctcaacacc
     5401 ccaatgctgt aacgcactaa gcatgtaaga gtgtaatgtg tgaagcgagc aagcccccaa
     5461 ggtatcaata gtataactgg agaagcctgt aaccgagtaa tatatgaatc agtcaagagt
     5521 ctaatagtcg aagcgagtaa cgtactaagt agcgaactac ataatgcgct aatctgagaa
     5581 tcgccgaata catcaacgtc ccaaccactg aacgtcgcaa tatgcgaata cccgaatcac
     5641 cgaacacggc aacactcgaa tctgcgaagc ggtcaaggtg ataactcgtc aacaggataa
     5701 gcgcctaagc tcctaagacg ctaagatagg aatgcctgaa tagcagaatc gcggaagcct
     5761 acaatgaccc aaggcggcaa tcgggtaatc ccggaaccgg agaatgctat aagtctacaa
     5821 gtaggtaatc tgctaacaca gtaatgcatg aagtatacaa ggagggaagg acagaatggg
     5881 acaaccgatg aagctagtaa gcgaggaatg cagtaagctc tcaagtgtag aactagccaa
     5941 gtccgcaact ctgcaagata tgaagaccgc aatggcgcaa cgtgacaatg gctgaactat
     6001 gcaacgcgcc aaccctctaa gagcccaacg gcggaatatc ctaaccgtat aatccgataa
     6061 gcgtagaact gcagaatctc ataacgtggt aaccgcctaa gtggagaata ctgtaactag
     6121 agaaccaccg aatggcataa tcctctaact aggtaaggcg ctaactgagg aaccccgtaa
     6181 ggtggcaagc agcgaatcta gtaaggtcct aagagaggaa cggccgaact gactaactag
     6241 acaacagcgc aagtcggcaa gcagtcaagg gctcaatagg ctaatatggg aagcccggaa
     6301 gtgaccaaga tctcaatccg tgaaccatac aactgcataa tggccgaagt ccggaacgcc
     6361 ggaagaggct aagcatagaa tacgccaatg tgagaaggta gcaaccatat aaccgcccaa
     6421 ccgtacaact catgaatgcc acaacatgac aaggactcaa cctacgaact actcaagtcc
     6481 ataaccgagg aacggatcaa gggagcaata ggcgaaggtg tcaacgtgtg aatgtagcaa
     6541 gtacgcaaga tcggaatctc ggaacagact aaggcatgaa gatgagaatg ggtcaagact
     6601 ggaataccgg aacgagccaa ccgtgtaagc gtataatgtg ataactgtag aacgcggcaa
     6661 tgagacaacc agataatgca ggaagctatc aagctcagaa gaggcgaatg caccaagcta
     6721 ctaatctccg aacaccgtaa cgtgggaata ccgcaacccg ctaatgcact aagcacctaa
     6781 gaggataacc cctcaatacg acaagtagac aagctaggaa tctcccaagc ctctaatagg
     6841 gtaagactcg aactacggaa gccctcaagt ccctaacgag tgaacagtcg aagtacggaa
     6901 cgctccaatg agtgaatcca ctaacccacg aagtatctaa ggacataata tgccaatgcg
     6961 ccaactgcct aatgtagtaa cgacccaaca tcctaagtca gtaagtgtac aagatgtcaa
     7021 tccagcaaca cggtaagagc gtaacggctc aagagcggaa ggccacaaca taccaaccat
     7081 agaaggtcgg aagagagcaa gcgatcaaga cgtcaacatc gtaatcatgt aagagatcaa
     7141 ctgggtaagt ccagaaccct cgaatcgtgg aactcggcaa cacctgaatg ggctaaccca
     7201 tcaagagtgg aagtcaggaa gatccgaacg tgagaatatc gtaagagctg aagcaccgaa
     7261 ggcgataata tcggaaggcg ggaagcgacg aagacactaa gtgcataatc gtataactat
     7321 gtaagatatc aaggcgccaa gtgggtaacg gtgtaagtag tgaaccggac aatgatagaa
     7381 ctacagaagt gcagaactat agaatcgctc aaccgctgaa tacctgaagc cgggaagctc
     7441 acaagggtct aagcgggcaa ctgagcaagt catgaagcgg gtaacgatag aagagactaa
     7501 ccagccaacg tccgaagtgt gtaataccct aataggtgaa ggacccaact agtgaacggc
     7561 ccaacagcct aacctggcaa cgtgccaagg tactaagtga ctaatggtct aagcgcgtaa
     7621 gtctagaatc tggtaagtac agaagcaccc aaccggtcaa catatgaagt ctgcaaggat
     7681 ggaaccggcc aacctcacaa ctgtgcaatc actgaaacgt tgaagcgtta cctgttaggt
     7741 aacgtagttg agctgtttca gtgacattga ccgggagcgc cctgtagcgg cgcattaagc
     7801 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7861 gctcccggga tcggaattcc ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7921 ggagtccacg ttctttaata gtggactctt gttccaaact ggaaaacgtc tagcggtatc
     7981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8101 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8521 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9841 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     9901 tatcacgagg cagaatttca gcctcc
//
