LOCUS       sequence-248649-       17450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector. Expresses YFP-Ezh2 fusion proteins in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17450)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ (M)-YFP-Ezh2
FEATURES             Location/Qualifiers
     source          1..17450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3008..3017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3014..3016,3017..3019,3020..3730)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3059..3080)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3320..3339)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3667..3688
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        8268..9479
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     9316..9335
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             9498..10205
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     misc_feature    10228..10800
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10395..10412)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10622..10641
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10803..11405
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     11302..11322
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11421..12009
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11474..11494)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11892..11903)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12216..12449
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(12550..12567)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12556..12780
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12826..13254
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12913..12932)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13123..13144
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13263..13283)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13268..13597
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13448..13583
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13510..13529
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    14805..14926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14975..14991)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14975..14991)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14988..15010)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14999..15015
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15023..15029,15030..15047,15048..15053))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15068..15089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15206..15223)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15377..15965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15457..15476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16136..16927,16928..16996))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16759..16778
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16997..17101)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    17149..17283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtccg
     3001 ctcgagggcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     3061 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     3121 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     3181 gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc
     3241 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     3301 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     3361 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     3421 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     3481 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     3541 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     3601 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
     3661 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     3721 gctgtacaag gcgaattcga tacgtatcga tggcgcgccc atgggccaga ctgggaagaa
     3781 atctgagaag ggaccagttt gttggcggaa gcgtgtaaaa tcagagtaca tgcgactgag
     3841 acagctcaag aggttcagac gagctgatga agtaaagagt atgtttagtt ccaatcgtca
     3901 gaaaattttg gaaagaacgg aaatcttaaa ccaagaatgg aaacagcgaa ggatacagcc
     3961 tgtgcacatc ctgacttctg tgagctcatt gcgcgggact agggagtgtt cggtgaccag
     4021 tgacttggat tttccaacac aagtcatccc attaaagact ctgaatgcag ttgcttcagt
     4081 acccataatg tattcttggt ctcccctaca gcagaatttt atggtggaag atgaaactgt
     4141 tttacataac attccttata tgggagatga agttttagat caggatggta ctttcattga
     4201 agaactaata aaaaattatg atgggaaagt acacggggat agagaatgtg ggtttataaa
     4261 tgatgaaatt tttgtggagt tggtgaatgc ccttggtcaa tataatgatg atgacgatga
     4321 tgatgatgga gacgatcctg aagaaagaga agaaaagcag aaagatctgg aggatcaccg
     4381 agatgataaa gaaagccgcc cacctcggaa atttccttct gataaaattt ttgaagccat
     4441 ttcctcaatg tttccagata agggcacagc agaagaacta aaggaaaaat ataaagaact
     4501 caccgaacag cagctcccag gcgcacttcc tcctgaatgt acccccaaca tagatggacc
     4561 aaatgctaaa tctgttcaga gagagcaaag cttacactcc tttcatacgc ttttctgtag
     4621 gcgatgtttt aaatatgact gcttcctaca tccttttcat gcaacaccca acacttataa
     4681 gcggaagaac acagaaacag ctctagacaa caaaccttgt ggaccacagt gttaccagca
     4741 tttggaggga gcaaaggagt ttgctgctgc tctcaccgct gagyggataa agaccccacc
     4801 aaaacgtcca ggaggccgca gaagaggacg gcttcccaat aacagtagca ggcccagcac
     4861 ccccaccatt aatgtgctgg aatcaaagga tacagacagt gatagggaag cagggactga
     4921 aacgggggga gagaacaatg ataaagaaga agaagagaag aaagatgaaa cttcgagctc
     4981 ctctgaagca aattctcggt gtcaaacacc aataaagatg aagccaaata ttgaacctcc
     5041 tgagaatgtg gagtggagtg gtgctgaagc ctcaatgttt agagtcctca ttggcactta
     5101 ctatgacaat ttctgtgcca ttgctaggtt aattgggacc aaaacatgta gacaggtgta
     5161 tgagtttaga gtcaaagaat ctagcatcat agctccagct cccgctgagg atgtggatac
     5221 tcctccaagg aaaaagaaga ggaaacaccg gttgtgggct gcacactgca gaaagataca
     5281 gctgaaaaag gacggctcct ctaaccatgt ttacaactat caaccctgtg atcatccacg
     5341 gcagccttgt gacagttcgt gcccttgtgt gatagcacaa aatttttgtg aaaagttttg
     5401 tcaatgtagt tcagagtgtc aaaaccgctt tccgggatgc cgctgcaaag cacagtgcaa
     5461 caccaagcag tgcccgtgct acctggctgt ccgagagtgt gaccctgacc tctgtcttac
     5521 ttgtggagcc gctgaccatt gggacagtaa aaatgtgtcc tgcaagaact gcagtattca
     5581 gcggggctcc aaaaagcatc tattgctggc accatctgac gtggcaggct gggggatttt
     5641 tatcaaagat cctgtgcaga aaaatgaatt catctcagaa tactgtggag agattatttc
     5701 tcaagatgaa gctgacagaa gagggaaagt gtatgataaa tacatgtgca gctttctgtt
     5761 caacttgaac aatgattttg tggtggatgc aacccgcaag ggtaacaaaa ttcgttttgc
     5821 aaatcattcg gtaaatccaa actgctatgc aaaagttatg atggttaacg gtgatcacag
     5881 gataggtatt tttgccaaga gagccatcca gactggcgaa gagctgtttt ttgattacag
     5941 atacagtcag gctgatgccc tgaagtatgt cggcatcgaa agagaaatgg aaatcccttg
     6001 acgacgcgtg gcgcgcccat gggccagact gggaagaaat ctgagaaggg accagtttgt
     6061 tggcggaagc gtgtaaaatc agagtacatg cgactgagac agctcaagag gttcagacga
     6121 gctgatgaag taaagagtat gtttagttcc aatcgtcaga aaattttgga aagaacggaa
     6181 atcttaaacc aagaatggaa acagcgaagg atacagcctg tgcacatcct gacttctgtg
     6241 agctcattgc gcgggactag ggagtgttcg gtgaccagtg acttggattt tccaacacaa
     6301 gtcatcccat taaagactct gaatgcagtt gcttcagtac ccataatgta ttcttggtct
     6361 cccctacagc agaattttat ggtggaagat gaaactgttt tacataacat tccttatatg
     6421 ggagatgaag ttttagatca ggatggtact ttcattgaag aactaataaa aaattatgat
     6481 gggaaagtac acggggatag agaatgtggg tttataaatg atgaaatttt tgtggagttg
     6541 gtgaatgccc ttggtcaata taatgatgat gacgatgatg atgatggaga cgatcctgaa
     6601 gaaagagaag aaaagcagaa agatctggag gatcaccgag atgataaaga aagccgccca
     6661 cctcggaaat ttccttctga taaaattttt gaagccattt cctcaatgtt tccagataag
     6721 ggcacagcag aagaactaaa ggaaaaatat aaagaactca ccgaacagca gctcccaggc
     6781 gcacttcctc ctgaatgtac ccccaacata gatggaccaa atgctaaatc tgttcagaga
     6841 gagcaaagct tacactcctt tcatacgctt ttctgtaggc gatgttttaa atatgactgc
     6901 ttcctacatc cttttcatgc aacacccaac acttataagc ggaagaacac agaaacagct
     6961 ctagacaaca aaccttgtgg accacagtgt taccagcatt tggagggagc aaaggagttt
     7021 gctgctgctc tcaccgctga gyggataaag accccaccaa aacgtccagg aggccgcaga
     7081 agaggacggc ttcccaataa cagtagcagg cccagcaccc ccaccattaa tgtgctggaa
     7141 tcaaaggata cagacagtga tagggaagca gggactgaaa cggggggaga gaacaatgat
     7201 aaagaagaag aagagaagaa agatgaaact tcgagctcct ctgaagcaaa ttctcggtgt
     7261 caaacaccaa taaagatgaa gccaaatatt gaacctcctg agaatgtgga gtggagtggt
     7321 gctgaagcct caatgtttag agtcctcatt ggcacttact atgacaattt ctgtgccatt
     7381 gctaggttaa ttgggaccaa aacatgtaga caggtgtatg agtttagagt caaagaatct
     7441 agcatcatag ctccagctcc cgctgaggat gtggatactc ctccaaggaa aaagaagagg
     7501 aaacaccggt tgtgggctgc acactgcaga aagatacagc tgaaaaagga cggctcctct
     7561 aaccatgttt acaactatca accctgtgat catccacggc agccttgtga cagttcgtgc
     7621 ccttgtgtga tagcacaaaa tttttgtgaa aagttttgtc aatgtagttc agagtgtcaa
     7681 aaccgctttc cgggatgccg ctgcaaagca cagtgcaaca ccaagcagtg cccgtgctac
     7741 ctggctgtcc gagagtgtga ccctgacctc tgtcttactt gtggagccgc tgaccattgg
     7801 gacagtaaaa atgtgtcctg caagaactgc agtattcagc ggggctccaa aaagcatcta
     7861 ttgctggcac catctgacgt ggcaggctgg gggattttta tcaaagatcc tgtgcagaaa
     7921 aatgaattca tctcagaata ctgtggagag attatttctc aagatgaagc tgacagaaga
     7981 gggaaagtgt atgataaata catgtgcagc tttctgttca acttgaacaa tgattttgtg
     8041 gtggatgcaa cccgcaaggg taacaaaatt cgttttgcaa atcattcggt aaatccaaac
     8101 tgctatgcaa aagttatgat ggttaacggt gatcacagga taggtatttt tgccaagaga
     8161 gccatccaga ctggcgaaga gctgtttttt gattacagat acagccaggc tgatgccctg
     8221 aagtatgtcg gcatcgaaag agaaatggaa atcccttgac gacgcgtggc ctccgcgccg
     8281 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     8341 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     8401 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     8461 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     8521 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     8581 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     8641 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     8701 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     8761 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     8821 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     8881 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     8941 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     9001 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     9061 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     9121 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     9181 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     9241 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     9301 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     9361 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     9421 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     9481 ctagcggatc cgccaccatg tctaggctgg acaagagcaa agtcataaac ggagctctgg
     9541 aattactcaa tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg
     9601 gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc
     9661 tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat
     9721 ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc tcacatcgcg
     9781 acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa
     9841 atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt
     9901 ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa
     9961 aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg
    10021 agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat
    10081 gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt
    10141 ttgacttaga catgctccca gccgatgccc ttgacgattt tgaccttgac atgctccccg
    10201 ggtaaggatc cgcggccgca aattccgccc ctctccctcc ccccccccta acgttactgg
    10261 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
    10321 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
    10381 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
    10441 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
    10501 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
    10561 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
    10621 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
    10681 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
    10741 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata
    10801 ccatggccac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
    10861 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
    10921 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
    10981 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
    11041 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg ctcgcgcatg gccgagttga
    11101 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
    11161 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
    11221 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgctggg gtgcccgcct
    11281 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
    11341 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
    11401 cctgagttcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg
    11461 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
    11521 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
    11581 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
    11641 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
    11701 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
    11761 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
    11821 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
    11881 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
    11941 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
    12001 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag
    12061 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
    12121 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
    12181 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg
    12241 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
    12301 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    12361 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    12421 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
    12481 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gtttaaaccc
    12541 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
    12601 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
    12661 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
    12721 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
    12781 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
    12841 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    12901 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
    12961 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
    13021 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
    13081 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
    13141 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
    13201 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
    13261 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    13321 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
    13381 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
    13441 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    13501 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
    13561 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
    13621 cattttcgga tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtctgtcga
    13681 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
    13741 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
    13801 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
    13861 cccgattccg gaagtgcttg acattgggga attaattcag cgagagcctg acctattgca
    13921 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
    13981 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
    14041 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
    14101 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
    14161 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
    14221 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
    14281 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
    14341 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
    14401 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
    14461 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
    14521 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
    14581 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
    14641 gacgccccag cactcgtccg agggcaaagg aatagcacgt gctacgagat ttcgattcca
    14701 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
    14761 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
    14821 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    14881 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
    14941 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    15001 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    15061 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    15121 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    15181 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    15241 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    15301 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    15361 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    15421 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    15481 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    15541 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    15601 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    15661 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    15721 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    15781 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    15841 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    15901 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    15961 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    16021 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    16081 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    16141 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    16201 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    16261 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    16321 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    16381 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    16441 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    16501 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    16561 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    16621 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    16681 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    16741 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    16801 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    16861 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    16921 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    16981 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    17041 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    17101 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt
    17161 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    17221 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    17281 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
    17341 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
    17401 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
