LOCUS       sequence-248651-        2061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes bsr (blasticidin-S deaminase) as a Type 3 part to be used in
            the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3_002
FEATURES             Location/Qualifiers
     source          1..2061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     245..264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             456..851
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     terminator      complement(871..905)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(976..1635)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1550..1569
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1817..2061,1..344)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
       61 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      121 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      181 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      241 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      301 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      361 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      421 cccctgattc tgtggataac cgtagtcggt ctcatatggc caagcctttg tctcaagaag
      481 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
      541 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
      601 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
      661 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
      721 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
      781 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
      841 tgtgggaggg cggatcctga gaccagacca ataaaaaacg cccggcggca accgagcgtt
      901 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
      961 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     1021 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     1081 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     1141 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     1201 aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     1261 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     1321 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     1381 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     1441 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     1501 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     1561 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     1621 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     1681 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa
     1741 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1801 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1861 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1921 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1981 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2041 tgttaccagt ggctgctgcc a
//
