LOCUS       sequence-248654-        2037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ble (Zeocin resistance) as a Type 3 part to be used in the 
            MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3_005
FEATURES             Location/Qualifiers
     source          1..2037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      117..705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     606..625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1208..1242)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1313..1972)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1887..1906
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
       61 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      241 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      361 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      421 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      481 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      541 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      601 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      661 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      721 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      781 tcccctgatt ctgtggataa ccgtagtcgg tctcatatgg ctaaattaac atctgccgtt
      841 cctgttttaa cagctaggga tgttgcaggt gctgtagagt tttggacaga taggttagga
      901 ttctcaagag actttgttga ggacgatttt gctggtgttg tcagggatga cgttacttta
      961 tttatctcag cagtccaaga tcaagttgtc cctgataata cattggcttg ggtctgggtc
     1021 aggggtttag atgaattata tgctgaatgg tcagaagttg tatctacaaa cttcagagat
     1081 gcttctggtc cagctatgac cgagattggt gaacagccat ggggtagaga atttgctttg
     1141 agagatccag ctggaaattg tgttcatttt gttgctgaag aacaagatgg atcctgagac
     1201 cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     1261 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
     1321 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     1381 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     1441 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     1501 aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa
     1561 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     1621 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     1681 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1741 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1801 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     1861 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     1921 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     1981 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttattt
//
