LOCUS       sequence-248657-        4059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes nuclear localized PHYB fused to activating domain of VP16 as
            Type 3 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3_009
FEATURES             Location/Qualifiers
     source          1..4059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     411..430
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      678..1266
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1167..1186
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3517..3750
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3751..3771
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(3791..3825)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(3896..4059,1..496))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
       61 aaacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
      121 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
      181 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
      241 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
      301 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
      361 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
      421 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
      481 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
      541 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca
      601 atcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
      661 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      721 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      781 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      841 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      901 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      961 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1021 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1081 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1141 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1201 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1261 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1321 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtagtcg gtctcatatg
     1381 gtttccggag tcgggggtag tggcggtggc cgtggcggtg gccgtggcgg agaagaagaa
     1441 ccgtcgtcaa gtcacactcc taataaccga agaggaggag aacaagctca atcgtcggga
     1501 acgaaatctc tcagaccaag aagcaacact gaatcaatga gcaaagcaat tcaacagtac
     1561 accgtcgacg caagactcca cgccgttttc gaacaatccg gcgaatcagg gaaatcattc
     1621 gactactcac aatcactcaa aacgacgacg tacggttcct ctgtacctga gcaacagatc
     1681 acagcttatc tctctcgaat ccagcgaggt ggttacattc agcctttcgg atgtatgatc
     1741 gccgtcgatg aatccagttt ccggatcatc ggttacagtg aaaacgccag agaaatgtta
     1801 gggattatgc ctcaatctgt tcctactctt gagaaacctg agattctagc tatgggaact
     1861 gatgtgagat ctttgttcac ttcttcgagc tcgattctac tcgagcgtgc tttcgttgct
     1921 cgagagatta ccttgttaaa tccggtttgg atccattcca agaatactgg taaaccgttt
     1981 tacgccattc ttcataggat tgatgttggt gttgttattg atttagagcc agctagaact
     2041 gaagatcctg cgctttctat tgctggtgct gttcaatcgc agaaactcgc ggttcgtgcg
     2101 atttctcagt tacaggctct tcctggtgga gatattaagc ttttgtgtga cactgtcgtg
     2161 gaaagtgtga gggacttgac tggttatgat cgtgttatgg tttataagtt tcatgaagat
     2221 gagcatggag aagttgtagc tgagagtaaa cgagacgatt tagagcctta tattggactg
     2281 cattatcctg ctactgatat tcctcaagcg tcaaggttct tgtttaagca gaaccgtgtc
     2341 cgaatgatag tagattgcaa tgccacacct gttcttgtgg tccaggacga taggctaact
     2401 cagtctatgt gcttggttgg ttctactctt agggctcctc atggttgtca ctctcagtat
     2461 atggctaaca tgggatctat tgcgtcttta gcaatggcgg ttataatcaa tggaaatgaa
     2521 gatgatggga gcaatgtagc tagtggaaga agctcgatga ggctttgggg tttggttgtt
     2581 tgccatcaca cttcttctcg ctgcataccg tttccgctaa ggtatgcttg tgagtttttg
     2641 atgcaggctt tcggtttaca gttaaacatg gaattgcagt tagctttgca aatgtcagag
     2701 aaacgcgttt tgagaacgca gacactgtta tgtgatatgc ttctgcgtga ctcgcctgct
     2761 ggaattgtta cacagagtcc cagtatcatg gacttagtga aatgtgacgg tgcagcattt
     2821 ctttaccacg ggaagtatta cccgttgggt gttgctccta gtgaagttca gataaaagat
     2881 gttgtggagt ggttgcttgc gaatcatgcg gattcaaccg gattaagcac tgatagttta
     2941 ggcgatgcgg ggtatcccgg tgcagctgcg ttaggggatg ctgtgtgcgg tatggcagtt
     3001 gcatatatca caaaaagaga ctttcttttt tggtttcgat ctcacactgc gaaagaaatc
     3061 aaatggggag gcgctaagca tcatccggag gataaagatg atgggcaacg aatgcatcct
     3121 cgttcgtcct ttcaggcttt tcttgaagtt gttaagagcc ggagtcagcc atgggaaact
     3181 gcggaaatgg atgcgattca ctcgctccag cttattctga gagactcttt taaagaatct
     3241 gaggcggcta tgaactctaa agttgtggat ggtgtggttc agccatgtag ggatatggcg
     3301 ggggaacagg ggattgatga gttaggtgaa ttcgatagtg ctggtagtgc tggtagtgct
     3361 ggttccgcgt acagccgcgc gcgtacgaaa aacaattacg ggtctaccat cgagggcctg
     3421 ctcgatctcc cggacgacga cgcccccgaa gaggcggggc tggcggctcc gcgcctgtcc
     3481 tttctccccg cgggacacac gcgcagactg tcgacggccc ccccgaccga tgtcagcctg
     3541 ggggacgagc tccacttaga cggcgaggac gtggcgatgg cgcatgccga cgcgctagac
     3601 gatttcgatc tggacatgtt gggggacggg gattccccgg gtccgggatt taccccccac
     3661 gactccgccc cctacggcgc tctggatatg gccgacttcg agtttgagca gatgtttacc
     3721 gatgcccttg gaattgacga gtacggtggg cccaagaaaa agcggaaggt gggatcctga
     3781 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     3841 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     3901 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     3961 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     4021 gcgtataata tttgcccatg gtgaaaacgg gggcgaaga
//
