LOCUS       sequence-248661-        3033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Flag-tagged ABIcs fused to GAL4 DNA binding domain as a Type
            3 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3_013
FEATURES             Location/Qualifiers
     source          1..3033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      131..719
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     620..639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             831..1271
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2175..2198
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      complement(2218..2252)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2323..2982)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2897..2916
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga
       61 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      241 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      781 tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggtctcat atgaagctac
      841 tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag
      901 aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa
      961 ccaaaaggtc accgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac
     1021 tggaacagct atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg
     1081 attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata
     1141 aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta acattgagac
     1201 agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt
     1261 tgactgtatc gacgcgtatg gtgcctttgt atggttttac ttcgatttgt ggaagaagac
     1321 ctgagatgga agctgctgtt tcgactatac caagattcct tcaatcttcc tctggttcga
     1381 tgttagatgg tcggtttgat cctcaatccg ccgctcattt cttcggtgtt tacgacggcc
     1441 atggcggttc tcaggtagcg aactattgta gagagaggat gcatttggct ttggcggagg
     1501 agatagctaa ggagaaaccg atgctctgcg atggtgatac gtggctggag aagtggaaga
     1561 aagctctttt caactcgttc ctgagagttg actcggagat tgagtcagtt gcgccggaga
     1621 cagttgggtc aacgtcggtg gttgccgttg ttttcccgtc acacatcttc gtcgctaact
     1681 gcggtgactc tagagccgtt ctttgccgcg gcaaaactgc acttccatta tccgttgacc
     1741 ataaaccgga tagagaagat gaagctgcga ggattgaagc cgcaggaggg aaagtgattc
     1801 agtggaatgg agctcgtgtt ttcggtgttc tcgccatgtc gagatccatt ggcgatagat
     1861 acttgaaacc atccatcatt cctgatccgg aagtgacggc tgtgaagaga gtaaaagaag
     1921 atgattgtct gattttggcg agtgacgggg tttgggatgt aatgacggat gaagaagcgt
     1981 gtgagatggc aaggaagcgg attctcttgt ggcacaagaa aaacgcggtg gctggggatg
     2041 catcgttgct cgcggatgag cggagaaagg aagggaaaga tcctgcggcg atgtccgcgg
     2101 ctgagtattt gtcaaagctg gcgatacaga gaggaagcaa agacaacata agtgtggtgg
     2161 tggttgattt gaaggattac aaggacgatg acgataaggg atcctgagac cagaccaata
     2221 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
     2281 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
     2341 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     2401 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     2461 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     2521 ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg
     2581 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     2641 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     2701 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     2761 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     2821 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     2881 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     2941 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     3001 tgaaaatctc gataactcaa aaaatacgcc cgg
//
