LOCUS       sequence-248662-        3465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Gq-coupled hM3D as a Type 3 part to be used in the MTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3_014
FEATURES             Location/Qualifiers
     source          1..3465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     500..519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             714..740
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      complement(2530..2564)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2635..3294)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3209..3228
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      661 tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggtctcat atgtacccat
      721 acgatgttcc agattacgct atgaccttgc acaataacag tacaacctcg cctttgtttc
      781 caaacatcag ctcctcctgg atacacagcc cctccgatgc agggctgccc ccgggaaccg
      841 tcactcattt cggcagctac aatgtttctc gagcagctgg caatttctcc tctccagacg
      901 gtaccaccga tgaccctctg ggaggtcata ccgtctggca agtggtcttc atcgctttct
      961 taacgggcat cctggccttg gtgaccatca tcggcaacat cctggtaatt gtgtcattta
     1021 aggtcaacaa gcagctgaag acggtcaaca actacttcct cttaagcctg gcctgtgccg
     1081 atctgattat cggggtcatt tcaatgaatc tgtttacgac ctacatcatc atgaatcgat
     1141 gggccttagg gaacttggcc tgtgacctct ggcttgccat tgactgcgta gccagcaatg
     1201 cctctgttat gaatcttctg gtcatcagct ttgacagata cttttccatc acgaggccgc
     1261 tcacgtaccg agccaaacga acaacaaaga gagccggtgt gatgatcggt ctggcttggg
     1321 tcatctcctt tgtcctttgg gctcctgcca tcttgttctg gcaatacttt gttggaaaga
     1381 gaactgtgcc tccgggagag tgcttcattc agttcctcag tgagcccacc attacttttg
     1441 gcacagccat cgctggtttt tatatgcctg tcaccattat gactatttta tactggagga
     1501 tctataagga aactgaaaag cgtaccaaag agcttgctgg cctgcaagcc tctgggacag
     1561 aggcagagac agaaaacttt gtccacccca cgggcagttc tcgaagctgc agcagttacg
     1621 aacttcaaca gcaaagcatg aaacgctcca acaggaggaa gtatggccgc tgccacttct
     1681 ggttcacaac caagagctgg aaacccagct ccgagcagat ggaccaagac cacagcagca
     1741 gtgacagttg gaacaacaat gatgctgctg cctccctgga gaactccgcc tcctccgacg
     1801 aggaggacat tggctccgaa acgagagcca tctactccat cgtgctcaag cttccgggtc
     1861 acagcaccat cctcaactcc accaagttac cctcatcgga caacctgcag gtgcctgagg
     1921 aggagctggg gatggtggac ttggagagga aagccgacaa gctgcaggcc cagaagagcg
     1981 tggacgatgg aggcagtttt ccaaaaagct tctccaagct tcccatccag ctagagtcag
     2041 ccgtggacac agctaagact tctgacgtca actcctcagt gggtaagagc acggccactc
     2101 tacctctgtc cttcaaggaa gccactctgg ccaagaggtt tgctctgaag accagaagtc
     2161 agatcactaa gcggaaaagg atgtccctgg tcaaggagaa gaaagcggcc cagaccctca
     2221 gtgcgatctt gcttgccttc atcatcactt ggaccccata caacatcatg gttctggtga
     2281 acaccttttg tgacagctgc atacccaaaa ccttttggaa tctgggctac tggctgtgct
     2341 acatcaacag caccgtgaac cccgtgtgct atgctctgtg caacaaaaca ttcagaacca
     2401 ctttcaagat gctgctgctg tgccagtgtg acaaaaaaaa gaggcgcaag cagcagtacc
     2461 agcagagaca gtcggtcatt tttcacaagc gcgcacccga gcaggccttg ggatcctgag
     2521 accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     2581 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     2641 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     2701 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     2761 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     2821 taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat
     2881 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     2941 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     3001 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     3061 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     3121 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     3181 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     3241 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     3301 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     3361 attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac
     3421 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatca
//
