LOCUS       sequence-248663-        3132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Gi-coupled hM4D as a Type 3 part to be used in the MTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3_015
FEATURES             Location/Qualifiers
     source          1..3132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     500..519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             714..740
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      complement(2197..2231)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2302..2961)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2876..2895
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      661 tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggtctcat atgtacccat
      721 acgatgttcc agattacgct atggccaact tcacacctgt caatggcagc tcgggcaatc
      781 agtccgtgcg cctggtcacg tcatcatccc acaatcgcta tgagactgtg gaaatggtct
      841 tcattgccac agtgacaggc tccctgagcc tggtgactgt cgtgggcaac atcctggtga
      901 tgctgtccat caaggtcaac aggcagctgc agacagtcaa caactacttc ctcttcagcc
      961 tggcgtgtgc tgatctcatc ataggcgcct tctccatgaa cctctacacc gtgtacatca
     1021 tcaagggcta ctggcccctg ggcgccgtgg tctgcgacct gtggctggcc ctggactgcg
     1081 tggtgagcaa cgcctccgtc atgaaccttc tcatcatcag ctttgaccgc tacttctgcg
     1141 tcaccaagcc tctcacctac cctgcccggc gcaccaccaa gatggcaggc ctcatgattg
     1201 ctgctgcctg ggtactgtcc ttcgtgctct gggcgcctgc catcttgttc tggcagtttg
     1261 tggtgggtaa gcggacggtg cccgacaacc agtgcttcat ccagttcctg tccaacccag
     1321 cagtgacctt tggcacagcc attgctggct tctacctgcc tgtggtcatc atgacggtgc
     1381 tgtacatcca catctccctg gccagtcgca gccgagtcca caagcaccgg cccgagggcc
     1441 cgaaggagaa gaaagccaag acgctggcct tcctcaagag cccactaatg aagcagagcg
     1501 tcaagaagcc cccgcccggg gaggccgccc gggaggagct gcgcaatggc aagctggagg
     1561 aggccccccc gccagcgctg ccaccgccac cgcgccccgt ggctgataag gacacttcca
     1621 atgagtccag ctcaggcagt gccacccaga acaccaagga acgcccagcc acagagctgt
     1681 ccaccacaga ggccaccacg cccgccatgc ccgcccctcc cctgcagccg cgggccctca
     1741 acccagcctc cagatggtcc aagatccaga ttgtgacgaa gcagacaggc aatgagtgtg
     1801 tgacagccat tgagattgtg cctgccacgc cggctggcat gcgccctgcg gccaacgtgg
     1861 cccgcaagtt cgccagcatc gctcgcaacc aggtgcgcaa gaagcggcag atggcggccc
     1921 gggagcgcaa agtgacacga acgatctttg ccattctgct agccttcatc ctcacctgga
     1981 cgccctacaa cgtcatggtc ctggtgaaca ccttctgcca gagctgcatc cctgacacgg
     2041 tgtggtccat tggctactgg ctctgctacg tcaacagcac catcaaccct gcctgctatg
     2101 ctctgtgcaa cgccaccttt aaaaagacct tccggcacct gctgctgtgc cagtatcgga
     2161 acatcggcac ggccagggga tcctgagacc agaccaataa aaaacgcccg gcggcaaccg
     2221 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     2281 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
     2341 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     2401 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     2461 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     2521 ggctgaaacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     2581 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     2641 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     2701 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
     2761 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     2821 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     2881 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     2941 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     3001 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc
     3061 gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3121 tagaaaagat ca
//
