LOCUS       sequence-248696-        3807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Absisic acid Receptor fused to VPR activation domain as a 
            Type 3 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3_034
FEATURES             Location/Qualifiers
     source          1..3807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     366..385
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1153..1302
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             1309..1329
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1759..2130
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             2149..2718
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      complement(2738..2772)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2843..3502)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3417..3436
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(3684..3807,1..465)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
       61 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      121 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      181 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      241 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      301 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      361 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      421 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      481 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      541 tcccctgatt ctgtggataa ccgtagtcgg tctcatatgg gtgggggcgc gccaactcaa
      601 gacgaattca cccaactctc ccaatcaatc gccgagttcc acacgtacca actcggtaac
      661 ggccgttgct catctctcct agctcagcga atccacgcgc cgccggaaac agtatggtcc
      721 gtggttagac gtttcgatag gccacagatt tacaaacact tcatcaaaag ctgtaacgtg
      781 agtgaagatt tcgagatgcg agtgggatgc acgcgcgacg tgaacgtgat aagtggatta
      841 ccggcgaata cttctcggga gagattagat ctgttggacg atgatcggag agtgactggg
      901 tttagtataa ccggtggtga acataggctg aggaattata aatcggttac gacggttcat
      961 agatttgaga aagaagaaga agaagaaagg atctggaccg ttgttttgga atcttatgtt
     1021 gttgatgtac cggaaggtaa ttcggaggaa gatacgagat tgtttgctga tacggttatt
     1081 agattgaatc ttcagaaact tgcttcgatc actgaagcta tgaacgaatt ctcaagcgct
     1141 gcaggtacta gtgatgcttt agacgatttt gacttagata tgcttggttc agacgcgtta
     1201 gacgacttcg acctagacat gttaggctca gatgcattgg acgacttcga tttagatatg
     1261 ttgggctccg atgccctaga tgactttgat ctagatatgc taggtagtcc caaaaagaag
     1321 aggaaagtgg gatcccagta tctgcccgac acagatgata gacaccgaat cgaagagaaa
     1381 cgcaagcgaa cgtatgaaac cttcaaatcg atcatgaaga aatcgccctt ctcgggtccg
     1441 accgatccca ggcccccacc gagaaggatt gcggtcccgt cccgctcgtc ggccagcgtg
     1501 ccgaagcctg cgccgcagcc ctaccccttc acgtcgagcc tgagcacaat caattatgac
     1561 gagttcccga cgatggtgtt cccctcggga caaatctcac aagcctcggc gctcgcacca
     1621 gcgcctcccc aagtccttcc gcaagcgcct gccccagcgc ctgcaccggc aatggtgtcc
     1681 gccctcgcac aggcccctgc gcccgtcccc gtgctcgcgc ctggaccgcc ccaggcggtc
     1741 gctccaccgg ctccgaagcc gacgcaggcc ggagagggaa cactctccga agcacttctt
     1801 caactccagt ttgatgacga ggatcttgga gcactccttg gaaactcgac agaccctgcg
     1861 gtgtttaccg acctcgcgtc agtagataac tccgaatttc agcagctttt gaaccagggt
     1921 atcccggtcg cgccacatac aacggagccc atgttgatgg aataccccga agcaatcacg
     1981 agacttgtga cgggagcgca gcggcctccc gatcccgcac ccgcaccttt gggggcacct
     2041 ggcctcccta acggactttt gagcggcgac gaggatttct cctccatcgc cgatatggat
     2101 ttctcagcct tgctgtcaca gatttccagc ggctctggca gcggcagccg ggattccagg
     2161 gaagggatgt ttttgccgaa gcctgaggcc ggctccgcta ttagtgacgt gtttgagggc
     2221 cgcgaggtgt gccagccaaa acgaatccgg ccatttcatc ctccaggaag tccatgggcc
     2281 aaccgcccac tccccgccag cctcgcacca acaccaaccg gtccagtaca tgagccagtc
     2341 gggtcactga ccccggcacc agtccctcag ccactggatc cagcgcccgc agtgactccc
     2401 gaggccagtc acctgttgga ggatcccgat gaagaaacga gccaggctgt caaagccctt
     2461 cgggagatgg ccgatactgt gattccccag aaggaagagg ctgcaatctg tggccaaatg
     2521 gacctttccc atccgccccc aaggggccat ctggatgagc tgacaaccac acttgagtcc
     2581 atgaccgagg atctgaacct ggactcaccc ctgaccccgg aattgaacga gattctggat
     2641 accttcctga acgacgagtg cctcttgcat gccatgcata tcagcacagg actgtccatc
     2701 ttcgacacat ctctgtttgg atcctgagac cagaccaata aaaaacgccc ggcggcaacc
     2761 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     2821 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta
     2881 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     2941 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     3001 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     3061 tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     3121 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     3181 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     3241 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
     3301 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     3361 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     3421 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     3481 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     3541 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     3601 cgatcaatca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3661 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3721 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3781 ctttttccga aggtaactgg cttcagc
//
